To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.
The protein content has been determined by a Bradford-based assay.
The PC12 nuclear extracts have been collected after a 30-minute incubation with or without forskolin (20 µM).
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Generated by Greene and Tischler (1976) from a transplantable rat adrenal pheochromocytoma line, PC12 cells respond reversibly to nerve growth factor (NGF) and therefore have a neuronal phenotype, making the line a useful model system for neuronal differentiation. They produce catecholamines (dopamine, norepinephrine), neurotransmitters, NPP and are therefore used in studies of differentiation, neurobiochemistry and neurobiology.
has not yet been referenced specifically in any publications.