特記事項To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.
The protein content has been determined by a Bradford-based assay.
The PC12 nuclear extracts have been collected after a 30-minute incubation with or without forskolin (20 µM).
保存方法Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
背景Generated by Greene and Tischler (1976) from a transplantable rat adrenal pheochromocytoma line, PC12 cells respond reversibly to nerve growth factor (NGF) and therefore have a neuronal phenotype, making the line a useful model system for neuronal differentiation. They produce catecholamines (dopamine, norepinephrine), neurotransmitters, NPP and are therefore used in studies of differentiation, neurobiochemistry and neurobiology.