Anti-RKIP (phospho S153) 抗体 [EP2845Y] (ab75971)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2845Y] to RKIP (phospho S153)
- Suitable for: WB
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-RKIP (phospho S153) antibody [EP2845Y]
RKIP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2845Y] to RKIP (phospho S153) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: C6 treated with 0.5uM TPA (12-O-tetradecanoylphorbol-13-acetate), HeLa treated with 100ng/ml PMA (Phorbol 12-myristate 13-acetate)
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2845Y -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75971の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
|
特記事項 |
---|
WB
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
ターゲット情報
-
機能
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor. -
配列類似性
Belongs to the phosphatidylethanolamine-binding protein family. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 5037 Human
- Entrez Gene: 29542 Rat
- Omim: 604591 Human
- SwissProt: P30086 Human
- SwissProt: P31044 Rat
- Unigene: 433863 Human
- Unigene: 29745 Rat
-
別名
- Epididymis luminal protein 210 antibody
- Epididymis secretory protein Li 34 antibody
- Epididymis secretory protein Li 96 antibody
see all
画像
-
Lane 1 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution (Purified)
Lane 2 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100ng/ml PMA (Phorbol 12-myristate 13-acetate) for 48 hours whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking/Diluting buffer: 5% NFDM/TBST
-
Lane 1 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution (Purified)
Lane 2 : Anti-RKIP (phospho S153) antibody [EP2845Y] (ab75971) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 2 : C6 (Rat glial tumor glial cell) treated with 0.5uM TPA (12-O-tetradecanoylphorbol-13-acetate) for 3 hours whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking/Diluting buffer: 5% NFDM/TBST
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (5)
ab75971 は 5 報の論文で使用されています。
- Wang Z et al. Phosphorylation at S153 as a Functional Switch of Phosphatidylethanolamine Binding Protein 1 in Cerebral Ischemia-Reperfusion Injury in Rats. Front Mol Neurosci 10:358 (2017). PubMed: 29163033
- Macrae K et al. Defining the role of DAG, mitochondrial function, and lipid deposition in palmitate-induced proinflammatory signaling and its counter-modulation by palmitoleate. J Lipid Res 54:2366-78 (2013). WB . PubMed: 23833248
- Al-Mulla F et al. Clinical implications for loss or diminution of expression of Raf-1 kinase inhibitory protein and its phosphorylated form in ductal breast cancer. Am J Cancer Res 3:446-64 (2013). WB, IHC-P ; Human . PubMed: 24224123
- Deiss K et al. Raf kinase inhibitor protein (RKIP) dimer formation controls its target switch from Raf1 to G protein-coupled receptor kinase (GRK) 2. J Biol Chem 287:23407-17 (2012). WB . PubMed: 22610096
- Litherland GJ et al. Protein kinase C isoforms zeta and iota mediate collagenase expression and cartilage destruction via STAT3- and ERK-dependent c-fos induction. J Biol Chem 285:22414-25 (2010). PubMed: 20463008