製品の概要

  • 製品名
    Anti-PBK antibody [EP2519Y]
    PBK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2519Y] to PBK
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    corresponding to Human PBK (N terminal).

  • ポジティブ・コントロール
    • HeLa cell lysate
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000 - 1/100000. Predicted molecular weight: 36 kDa.
IP 1/30.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • ターゲット情報

    • 機能
      Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
    • 組織特異性
      Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
    • 配列類似性
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Phosphorylated; in a cell-cycle dependent manner at mitosis.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cancer/testis antigen 84 antibody
      • CT84 antibody
      • Epididymis luminal protein 164 antibody
      • FLJ14385 antibody
      • HEL164 antibody
      • Lymphokine activated killer T cell originated protein kinase antibody
      • Lymphokine-activated killer T-cell-originated protein kinase antibody
      • MAPKK like protein kinase antibody
      • MAPKK-like protein kinase antibody
      • Nori 3 antibody
      • Nori-3 antibody
      • Nori3 antibody
      • PBK antibody
      • PDZ binding kinase antibody
      • PDZ-binding kinase antibody
      • Serine/threonine protein kinase antibody
      • Spermatogenesis related protein kinase antibody
      • Spermatogenesis-related protein kinase antibody
      • SPK antibody
      • T LAK cell originated protein kinase antibody
      • T-LAK cell-originated protein kinase antibody
      • TOPK antibody
      • TOPK_HUMAN antibody
      see all

    画像



    • Predicted band size : 36 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: PBK knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Human tonsil tissue lysate (20 µg)
      Lanes 1 to 4: Merged signal (red and green). Green - ab75983 observed at 40 kDa. Red - loading control, ab7291, observed at 52 kDa.
      ab75983 was shown to recognize PBK when PBK knockout samples were used, along with additional cross-reactive bands. Wild-type and PBK knockout samples were subjected to SDS-PAGE. ab75983 at a dilution of 1/5000 and ab7291 (loading control to alpha Tubulin) at a dilution of 1/1000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-PBK antibody [EP2519Y] (ab75983) at 1/100000 dilution + HeLa cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 36 kDa
      Observed band size : 36 kDa

    参考文献

    ab75983 has not yet been referenced specifically in any publications.

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