製品の概要

  • 製品名
    Anti-Paxillin (phospho Y181) antibody [EP41(2)Y]
    Paxillin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP41(2)Y] to Paxillin (phospho Y181)
  • 特異性
    ab76235 only detects Paxillin phosphorylated on Tyrosine 181.
  • アプリケーション
    適用あり: WB, IP, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phosphopeptide corresponding to residues surrounding Tyrosine 181 of Human Paxillin.

  • ポジティブ・コントロール
    • HeLa cell lysate treated with pervanadate.
  • 特記事項

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 64 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
  • 配列類似性
    Belongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • 翻訳後修飾
    Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
  • 細胞内局在
    Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ16691 antibody
    • FLJ23042 antibody
    • Paired box protein Pax 1 antibody
    • PAX 1 antibody
    • PAX1 antibody
    • PAXI_HUMAN antibody
    • Paxillin alpha antibody
    • Paxillin antibody
    • PXN antibody
    • PXN protein antibody
    see all

画像

  • All lanes : Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] (ab76235) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 64 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

参考文献

This product has been referenced in:
  • Yanagishita T  et al. Actin-binding protein, Espin: a novel metastatic regulator for melanoma. Mol Cancer Res 12:440-6 (2014). WB ; Mouse . Read more (PubMed: 24337068) »
  • Kim SJ  et al. Galectin-3 facilitates cell motility in gastric cancer by up-regulating protease-activated receptor-1 (PAR-1) and matrix metalloproteinase-1 (MMP-1). PLoS One 6:e25103 (2011). WB ; Human . Read more (PubMed: 21966428) »

See all 2 Publications for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab76235.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録