製品の概要

  • 製品名Anti-Paxillin (phospho Y181) antibody [EP41(2)Y]
    Paxillin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP41(2)Y] to Paxillin (phospho Y181)
  • 特異性ab76235 only detects Paxillin phosphorylated on Tyrosine 181.
  • アプリケーション適用あり: WB, IP, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phosphopeptide corresponding to residues surrounding Tyrosine 181 of Human Paxillin.

  • ポジティブ・コントロール
    • HeLa cell lysate treated with pervanadate.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 64 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
  • 配列類似性Belongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • 翻訳後修飾Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
  • 細胞内局在Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ16691 antibody
    • FLJ23042 antibody
    • Paired box protein Pax 1 antibody
    • PAX 1 antibody
    • PAX1 antibody
    • PAXI_HUMAN antibody
    • Paxillin alpha antibody
    • Paxillin antibody
    • PXN antibody
    • PXN protein antibody
    see all

Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] 画像

  • All lanes : Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] (ab76235) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 64 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] (ab76235) 使用論文

This product has been referenced in:
  • Yanagishita T  et al. Actin-binding protein, Espin: a novel metastatic regulator for melanoma. Mol Cancer Res 12:440-6 (2014). WB ; Mouse . Read more (PubMed: 24337068) »
  • Kim SJ  et al. Galectin-3 facilitates cell motility in gastric cancer by up-regulating protease-activated receptor-1 (PAR-1) and matrix metalloproteinase-1 (MMP-1). PLoS One 6:e25103 (2011). WB ; Human . Read more (PubMed: 21966428) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab76235.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"