保存方法 Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
バッファー Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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精製度 Immunogen affinity purified
特記事項（精製） ab75740 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
1/50 - 1/100.
機能 Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
配列類似性 Belongs to the paxillin family. Contains 4 LIM zinc-binding domains.
翻訳後修飾 Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
細胞内局在 Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
Information by UniProt
Paired box protein Pax 1 antibody
Anti-Paxillin (phospho Y118) antibody 画像
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Paxillin (phospho Y118) antibody (ab75740)
ab75740 at 1/50 dilution staining Paxillin in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
Western blot - Paxillin (phospho Y118) antibody (ab75740)
All lanes : Anti-Paxillin (phospho Y118) antibody (ab75740) at 1/500 dilution Lane 1 : HeLa cell extract, untreated Lane 2 : HeLa cell extract treated with EGF Predicted band size : 64 kDa Observed band size : 64 kDa
Anti-Paxillin (phospho Y118) antibody (ab75740) 使用論文
This product has been referenced in:
et al. Src Family Kinases Modulate the Loss of Endothelial Barrier Function in Response to TNF-a: Crosstalk with p38 Signaling. PLoS One 11:e0161975 (2016).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"