製品の概要

  • 製品名
    Anti-Paxillin (phospho S126) antibody
    Paxillin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Paxillin (phospho S126)
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken
  • 免疫原

    Chemically synthesized phosphopeptide derived from the region of human paxillin that contains serine 126.

  • ポジティブ・コントロール
    • RAW 264.7 cells treated with LPS.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS(without Mg2+ and Ca2+), 1mg/ml BSA(IgG, protease free). pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at serine 126.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab24402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 68 kDa.

ターゲット情報

画像

  • All lanes : Anti-Paxillin (phospho S126) antibody (ab24402) at 1/1000 dilution

    Lane 1 : Lysate from untreated RAW 264.7 cells
    Lane 2 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes
    Lane 3 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with non-phosphorylated peptide corresponding to the immunogen
    Lane 4 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with generic phosphoserine-containing peptide
    Lane 5 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with phosphopeptide corresponding to Paxillin [pS91 ]
    Lane 6 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with phosphopeptide immunogen

    Secondary
    Goat F(ab)2 anti-rabbit IgG HRP conjugate.

    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • Boeckeler K  et al. Manipulating signal delivery - plasma-membrane ERK activation in aPKC-dependent migration. J Cell Sci 123:2725-32 (2010). WB ; Rat . Read more (PubMed: 20647370) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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