製品の概要

  • 製品名Anti-Paxillin antibody
    Paxillin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Paxillin
  • アプリケーション適用あり: WB, IHC-Fr, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken
  • 免疫原

    Synthetic peptide derived from residues 1 - 100 of Mouse Paxillin.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in NIH3T3 whole cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab2264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 68 kDa.
IHC-Fr Use at an assay dependent concentration. PubMed: 22629443
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100.

ターゲット情報

Anti-Paxillin antibody 画像

  • Anti-Paxillin antibody (ab2264) at 1/500 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ab2264 staining human and mouse pancreas and rat testis tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential (prediluted) peroxidase and protein block for 20 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C.  A HRP-conjugated goat anti-rabbit anitbody was used as the secondary.

    See Abreview

  • ab2264 staining human U2OS, mouse MEF, and rat RIE1 cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton-100 [PBS] prior to blocking in 1% BSA for 15 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C.  The secondary antibody was an Alexa Fluor® 647 conjugated donkey anti-rabbit, diluted 1/1000.

    See Abreview

  • Immunohistochemical analysis of frozen sections of frontonasal processes from wild-type (control) or RockDN;Wnt1-cre mutant (RockDN) mouse embyros; Paxillin was stained with ab2264.

Anti-Paxillin antibody (ab2264) 使用論文

This product has been referenced in:
  • Li A  et al. The interactome of LIM domain proteins: the contributions of LIM domain proteins to heart failure and heart development. Proteomics 12:203-25 (2012). Read more (PubMed: 22253135) »
  • Phillips HM  et al. Neural crest cell survival is dependent on Rho kinase and is required for development of the mid face in mouse embryos. PLoS One 7:e37685 (2012). IHC-Fr ; Mouse . Read more (PubMed: 22629443) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MDA-MB-435S)
Specification MDA-MB-435S
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
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Abcam user community

Verified customer

投稿 Feb 28 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (U2OS)
Total protein in input 400 µg
Specification U2OS
Immuno-precipitation step Protein A
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Mr. Antibody Solutions

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投稿 Apr 28 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human (U2OS) mouse (MEF) rat (RIE1))
Specification Human (U2OS) mouse (MEF) rat (RIE1)
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton-100 [PBS]
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
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Mr. Antibody Solutions

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投稿 Apr 10 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human and mouse (pancreas), rat (testis))
Specification human and mouse (pancreas), rat (testis)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization No
Blocking step Sequential (prediluated) peroxidase and protein block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
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Mr. Antibody Solutions

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投稿 Apr 08 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS, NIH-3T3, Raw264.7, PC12)
Loading amount 20 µg
Specification U2OS, NIH-3T3, Raw264.7, PC12
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Verified customer

投稿 Apr 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"