Anti-PAX9 抗体 [7C2] (ab28538)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [7C2] to PAX9
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-PAX9 antibody [7C2]
PAX9 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [7C2] to PAX9 -
由来種
Rat -
特異性
Does not cross react with other PAX proteins -
アプリケーション
適用あり: ICC/IF, WBmore details
適用なし: IHC-P -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment within Mouse PAX9 aa 250 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- WB: Mouse esophagus, Mouse skeletal muscle tissue. ICC/IF: NIH/3T3 cells.
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特記事項
This product has switched from a hybridoma to recombinant production method on 08th March 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
7C2 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28538の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/20.
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WB |
1/1000. Predicted molecular weight: 38 kDa.
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特記事項 |
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ICC/IF
1/20. |
WB
1/1000. Predicted molecular weight: 38 kDa. |
ターゲット情報
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機能
Transcription factor required for normal development of thymus, parathyroid glands, ultimobranchial bodies, teeth, skeletal elements of skull and larynx as well as distal limbs. -
関連疾患
Defects in PAX9 are the cause of tooth agenesis selective type 3 (STHAG3) [MIM:604625]. A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth). -
配列類似性
Contains 1 paired domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 18511 Mouse
- SwissProt: P47242 Mouse
- Unigene: 5035 Mouse
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別名
- Paired box 9 antibody
- Paired box gene 9 antibody
- Paired box homeotic gene 9 antibody
see all
画像
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All lanes : Anti-PAX9 antibody [7C2] (ab28538) at 1/1000 dilution
Lane 1 : Mouse esophagus tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 38 kDa
Exposure time: 70 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling PAX9 with ab28538 at 1/20 (49.7 µg/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear staining in NIH/3T3 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab28538 は 11 報の論文で使用されています。
- Pinzon-Guzman C et al. Noggin regulates foregut progenitor cell programming, and misexpression leads to esophageal atresia. J Clin Invest 130:4396-4410 (2020). PubMed: 32427591
- Koterazawa Y et al. Retinoic acid receptor ? activation promotes differentiation of human induced pluripotent stem cells into esophageal epithelium. J Gastroenterol 55:763-774 (2020). PubMed: 32556644
- Yuan Y et al. Spatiotemporal cellular movement and fate decisions during first pharyngeal arch morphogenesis. Sci Adv 6:N/A (2020). PubMed: 33328221
- Phillips HM et al. Pax9 is required for cardiovascular development and interacts with Tbx1 in the pharyngeal endoderm to control 4th pharyngeal arch artery morphogenesis. Development 146:N/A (2019). PubMed: 31444215
- Bar C et al. Polycomb Repressive Complex 1 Controls Maintenance of Fungiform Papillae by Repressing Sonic Hedgehog Expression. Cell Rep 28:257-266.e5 (2019). PubMed: 31269445