製品の概要

  • 製品名Anti-PAX9 antibody [7C2]
    PAX9 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [7C2] to PAX9
  • 特異性Does not cross react with other PAX proteins
  • アプリケーション適用あり: IP, IHC-Fr, ICC/IF, IHC-P, WB, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant PAX9-MBP fusion protein (Mouse)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28538 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa.
Flow Cyt Use 1µg for 106 cells. ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Transcription factor required for normal development of thymus, parathyroid glands, ultimobranchial bodies, teeth, skeletal elements of skull and larynx as well as distal limbs.
  • 関連疾患Defects in PAX9 are the cause of tooth agenesis selective type 3 (STHAG3) [MIM:604625]. A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth).
  • 配列類似性Contains 1 paired domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Paired box 9 antibody
    • Paired box gene 9 antibody
    • Paired box homeotic gene 9 antibody
    • Paired box protein 9 antibody
    • Paired box protein Pax 9 antibody
    • Paired box protein Pax-9 antibody
    • Paired box protein Pax9 antibody
    • Paired domain gene 9 antibody
    • PAX 9 antibody
    • PAX9 antibody
    • PAX9_HUMAN antibody
    • STHAG3 antibody
    see all

Anti-PAX9 antibody [7C2] 画像

  • ab28538 staining PAX9 in mouse E11.5 embryo tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 2% paraformaldehyde in PBS for 2 hours at 4°C then placed in 30% sucrose overnight at 4°C prior to embedding in OCT using liquid nitrogen. Tissue was blocked with 1X PBS + 1.5% Milk + 1.5% BSA + 0.1% Triton X-100 for 12 hours at 4°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 1 hour at 23°C. An Alexa Fluor® 568-conjugated goat anti-rat IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • Staining of PAX9 protein in human oesophageal epithelium. Magnification x200
  • All lanes : Anti-PAX9 antibody [7C2] (ab28538)

    Lane 1 : mouse oesophageal protein extract
    Lane 2 : human oesophageal protein extract


    Predicted band size : 38 kDa
    Observed band size : 38 kDa
  • ICC/IF image of ab28538 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28538, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HepG2 cells stained with ab28538 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28538, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was goat anti-rat DyLight® 488  (IgG H+L) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG1, kappa monoclonal [RTK2071] (ab18412, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-PAX9 antibody [7C2] (ab28538) 使用論文

This product has been referenced in:
  • Sherwood RI  et al. Transcriptional dynamics of endodermal organ formation. Dev Dyn 238:29-42 (2009). ICC/IF ; Mouse . Read more (PubMed: 19097184) »
  • Kendall J  et al. Oncogenic cooperation and coamplification of developmental transcription factor genes in lung cancer. Proc Natl Acad Sci U S A 104:16663-8 (2007). Read more (PubMed: 17925434) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E11.5 embryos)
Permeabilization No
Specification E11.5 embryos
Blocking step PBSMBT (1X PBS, 1.5% Milk, 1.5% BSA, 0.1% Triton X-100) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Mr. William Wood

Verified customer

投稿 Apr 30 2014

Here is the IHC protocol I received from the laboratory that validated the antibody for this application. Please let me know if you have any questions. As I mentioned in my previous e-mail, if your protocol is similar to this one, I will be happy to di...

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Thank you for your enquiry and your interest.

We have several antibodies against PAX9 but none of them have been tested specifically in ChIP application. Here are some which recognize mouse (murine) PAX9 and detect the native form of the prot...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"