製品の概要

  • 製品名Anti-PAX8 antibody [PAX8R1]
    PAX8 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PAX8R1] to PAX8
  • アプリケーション適用あり: Flow Cyt, IHC-P, WB, IP, ICC, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant C-terminal fragment corresponding to Human PAX8

  • ポジティブ・コントロール
    • HeLa, NIH3T3 or F2408 nuclear cell lysate HeLa cells RIPA lysate of HeLa cells
  • 特記事項

    IHC-Fr and IHC-P are not batch-tested and therefore variability between batches might be seen for these applications.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

0.5-2 µg/sample





ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 48 kDa.
IP Use at an assay dependent concentration.

1-5 µg/sample

ICC Use a concentration of 2 - 100 µg/ml.
IHC-Fr 1/20.

ターゲット情報

  • 機能Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
  • 組織特異性Expressed in the excretory system, thyroid gland and Wilms tumors.
  • 関連疾患Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
  • 配列類似性Contains 1 paired domain.
  • 発生段階In developing excretory system, during thyroid differentiation and in adult thyroid.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • OTTHUMP00000158659 antibody
    • OTTHUMP00000158660 antibody
    • OTTHUMP00000203723 antibody
    • OTTHUMP00000203724 antibody
    • Paired box 8 antibody
    • Paired box gene 8 antibody
    • paired box homeotic gene 8 antibody
    • Paired box protein Pax 8 antibody
    • Paired box protein Pax-8 antibody
    • Paired domain gene 8 antibody
    • PAX 8 antibody
    • PAX8 antibody
    • PAX8_HUMAN antibody
    see all

Anti-PAX8 antibody [PAX8R1] 画像

  • ab53490 staining PAX8 in Mouse thyroid tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Tween 20 and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/20 in 10% goat serum in PBS) for 1 hour at 24°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 48 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 48 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + F2408 whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 48 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab53490, at a 1/10 dilution, staining PAX8 in HeLa cells, using Immunocytochemistry, plus AlexaFluor®488 Goat Anti-mouse IgG secondary anitbody.
  • Immunoprecipitation: ab53490: 5µg, RIPA lysate of HeLa cells: 500µg. WB: ab53490 at a 1:100 dilution, Secondary antibody - anti-mouse IgG at a 1:1000 dilution
  • HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with ab53490 at 0.5 µg/sample (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488-conjugated goat anti-mouse IgG (0.25µg)

  • IHC image of ab53490 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53490, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PAX8 antibody [PAX8R1] (ab53490) 使用論文

This product has been referenced in:
  • Wang M  et al. PAX2 and PAX8 reliably distinguishes ovarian serous tumors from mucinous tumors. Appl Immunohistochem Mol Morphol 23:280-7 (2015). Read more (PubMed: 24992169) »
  • Garreta E  et al. Low oxygen tension enhances the generation of lung progenitor cells from mouse embryonic and induced pluripotent stem cells. Physiol Rep 2:N/A (2014). ICC/IF ; Mouse . Read more (PubMed: 25347858) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Mouse Tissue sections (mouse thyroid)
Specification mouse thyroid
Permeabilization Yes - 0.1% Tween 20
Fixative Paraformaldehyde
Username

Mr. Sanjay Gawade

Verified customer

投稿 Jun 26 2013

Thank you for contacting us.

I am sorry to hear that you are also experiencing problems with this antibody (additionally to the Anti-TTF1 antibody [8G7G3/1] (ab72876) ). Could you please fill in the questionnaire for both products and send the...

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Thank you for contacting us. Although we have indicated Pax8 to be located in the nucleus, the literature shows Pax8 expression can be nuclear as well as cytoplasmic. I apologize for the confusion.

I hope this information is helpful to you. Pl...

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Thank you for calling Abcam earlier today.

Please find the link to the concentration calculator that we discussed below:

https://www.msu.edu/˜nixonjos/more/dilution.html?stock=1&needed=0.0001&volume=1&total=0.1

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Thank you for your patience.

I have heard back from the lab with the following information:

We have not done anytesting in regards to cross reactivity of ab53490 with PAX2 and PAX5 on endogenous protein. Also, we haven't done any e...

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Thank you for contacting us.

The immunogen is a recombinant C-terminal fragment corresponding to Human PAX8,based on theamino acid range 318-426 aa. The exact epitope has not been mapped, therefore we do not know where the antibody would bin...

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I'm happy to help! Best of luck with your experiment.

I have used these columns but not for an application as sensitive as the one you describe.For my purposes it had been sufficient to have perhaps 95% or more of protein that should flow through will flow through and about the same percentage of protein ...

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Thank you for your enquiry.

We only have the one formulation of ab53490, which contains 1% BSA. We can often create a custom batch with customer buffer formulations but this would require a bulk order, which would be 10 vials of antibody. Read More

Thank you for contacting us. I am sorry that ab53490 has given no signal on your customer's samples. Since the PAX8 antigen is expressed in the nucleus, adding a brief permeabilization step may help to increase the signal. I would recommend using 0.1...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"