製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PAX8
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human PAX8.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human thyroid tissue lysate as well as the following whole cell lysates: HEK293; A498; LnCaP.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

ターゲット情報

  • 機能
    Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
  • 組織特異性
    Expressed in the excretory system, thyroid gland and Wilms tumors.
  • 関連疾患
    Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
  • 配列類似性
    Contains 1 paired domain.
  • 発生段階
    In developing excretory system, during thyroid differentiation and in adult thyroid.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • OTTHUMP00000158659 antibody
    • OTTHUMP00000158660 antibody
    • OTTHUMP00000203723 antibody
    • OTTHUMP00000203724 antibody
    • Paired box 8 antibody
    • Paired box gene 8 antibody
    • paired box homeotic gene 8 antibody
    • Paired box protein Pax 8 antibody
    • Paired box protein Pax-8 antibody
    • Paired domain gene 8 antibody
    • PAX 8 antibody
    • PAX8 antibody
    • PAX8_HUMAN antibody
    see all

画像

  • All lanes : Anti-PAX8 antibody (ab95217) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 3 : LNCaP nuclear extract lysate (ab14857)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 110 kDa,41 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

プロトコール

参考文献

ab95217 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Nov 07 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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