製品の概要

  • 製品名Anti-PAX7 antibody
    PAX7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PAX7
  • アプリケーション適用あり: WB, ICC/IF, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human PAX7.

    (Peptide available as ab37576.)

  • ポジティブ・コントロール
    • ab34360 was positive in the following Whole Cell Lysates: TE671 (Human Rhabdomyosarcoma cell line).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab34360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 20723755
IHC-Fr Use at an assay dependent concentration. PubMed: 22253929

ターゲット情報

  • 機能Probable transcription factor. May have a role in myogenesis.
  • 関連疾患Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator.
  • 配列類似性Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 paired domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ37460 antibody
    • HUP1 antibody
    • OTTHUMP00000002534 antibody
    • Paired box 7 antibody
    • Paired box gene 7 antibody
    • Paired box homeotic gene 7 antibody
    • Paired box protein Pax-7 antibody
    • Paired domain gene 7 antibody
    • Paired domain gene HuP1 antibody
    • Pax7 antibody
    • PAX7 transcriptional factor antibody
    • PAX7/FKHR fusion gene, included antibody
    • PAX7_HUMAN antibody
    • PAX7B antibody
    • RGD1564360 antibody
    • RMS2 antibody
    see all

Anti-PAX7 antibody 画像

  • All lanes : Anti-PAX7 antibody (ab34360) at 1 µg/ml

    Lane 1 : Human Uterus Tissue Lysate (negative control)
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.
    Blocking peptides at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab34360 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

Anti-PAX7 antibody (ab34360) 使用論文

This product has been referenced in:
  • MacKrell JG  et al. Molecular targets of androgen signaling that characterize skeletal muscle recovery and regeneration. Nucl Recept Signal 13:e005 (2015). Read more (PubMed: 26457071) »
  • Gigliotti D  et al. Atrophy, inducible satellite cell activation, and possible denervation of supraspinatus muscle in injured human rotator-cuff muscle. Am J Physiol Cell Physiol 309:C383-91 (2015). Read more (PubMed: 26135801) »

See all 12 Publications for this product

Product Wall

Thank you for contacting us.

Unfortunately, we do not carry a conjugated antibody for PAX7.

However, we carry a number of easy-to-use conjugation kits. Please see the link for the list of kits we carry:

http://www.abcam.c...

Read More

Thank you for your phone call.

I am going to contact the lab shortly regarding ab47052.

The link to our EasyLink conjugation kits can be found here: http://www.abcam.com/Easylink

For the information regarding the discount ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (primary mouse cells)
Loading amount 20 µg
Specification primary mouse cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 19 2010

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (muscle sections)
Specification muscle sections
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100 0.1% in PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Username

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Verified customer

投稿 Jun 30 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (liver (human mouse and rat) and mouse small bowel)
Specification liver (human mouse and rat) and mouse small bowel
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization No
Blocking step Sequential (prediluated) peroxidase and protein block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Jun 04 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa (Hu), HEK293 (Hu), NIH-3T3, PC-12)
Loading amount 20 µg
Specification HeLa (Hu), HEK293 (Hu), NIH-3T3, PC-12
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Apr 07 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"