製品の概要

  • 製品名Anti-PAX5 antibody [mAbcam52588]
    PAX5 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [mAbcam52588] to PAX5
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PAX5.

    (Peptide available as ab55851.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Raji and HEK293 Whole Cell Lysates

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
  • 関連疾患A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
    Leukemia, acute lymphoblastic, 3
  • 配列類似性Contains 1 paired domain.
  • 発生段階Expressed at early B-cell differentiation, in the developing CNS and in adult testis.
  • 翻訳後修飾O-glycosylated.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B cell lineage specific activator antibody
    • B cell lineage specific activator protein antibody
    • B cell specific activator protein antibody
    • B cell specific transcription factor antibody
    • B-cell-specific transcription factor antibody
    • BSAP antibody
    • EBB-1 antibody
    • KLP antibody
    • Paired box 5 antibody
    • Paired box gene 5 (B cell lineage specific activator protein) antibody
    • Paired box gene 5 (B cell lineage specific activator) antibody
    • Paired box gene 5 antibody
    • Paired box homeotic gene 5 antibody
    • Paired box protein Pax 5 antibody
    • Paired box protein Pax-5 antibody
    • Paired domain gene 5 antibody
    • PAX 5 antibody
    • PAX5 antibody
    • PAX5_HUMAN antibody
    • Transcription factor PAX 5 antibody
    see all

Anti-PAX5 antibody [mAbcam52588] 画像

  • All lanes : Anti-PAX5 antibody [mAbcam52588] (ab52588) at 1 µg/ml

    Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate (blocked with 1% milk)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (blocked with 1% Milk)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Overlay histogram showing Ramos cells stained with ab52588 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52588, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-PAX5 antibody [mAbcam52588] (ab52588) 使用論文

ab52588 has not yet been referenced specifically in any publications.

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