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RabMAb

Anti-PAX5 抗体 [EPR3729] (ab92512)

製品の概要

  • 製品名
    Anti-PAX5 antibody [EPR3729]
    PAX5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3729] to PAX5
  • アプリケーション
    適用あり: WB, ICC, Flow Cytmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human PAX5.

  • ポジティブ・コントロール
    • Ramos and Daudi cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC
Flow Cyt
  • 追加情報
    ICC: 1/100 - 1/250.
    WB: 1/1000 - 1/10,000. Predicted molecular weight: 42 kDa.

    Is unsuitable for Flow Cyt, IHC-P or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
    • 関連疾患
      A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
      Leukemia, acute lymphoblastic, 3
    • 配列類似性
      Contains 1 paired domain.
    • 発生段階
      Expressed at early B-cell differentiation, in the developing CNS and in adult testis.
    • 翻訳後修飾
      O-glycosylated.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • B cell lineage specific activator antibody
      • B cell lineage specific activator protein antibody
      • B cell specific activator protein antibody
      • B cell specific transcription factor antibody
      • B-cell-specific transcription factor antibody
      • BSAP antibody
      • EBB-1 antibody
      • KLP antibody
      • Paired box 5 antibody
      • Paired box gene 5 (B cell lineage specific activator protein) antibody
      • Paired box gene 5 (B cell lineage specific activator) antibody
      • Paired box gene 5 antibody
      • Paired box homeotic gene 5 antibody
      • Paired box protein Pax 5 antibody
      • Paired box protein Pax-5 antibody
      • Paired domain gene 5 antibody
      • PAX 5 antibody
      • PAX5 antibody
      • PAX5_HUMAN antibody
      • Transcription factor PAX 5 antibody
      see all

    Anti-PAX5 antibody [EPR3729] 画像

    • All lanes : Anti-PAX5 antibody [EPR3729] (ab92512) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : Daudi cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 42 kDa
    • Flow Cytometry analysis of Daudi (human Burkitt's lymphoma) cells labeling PAX5 with unpurified ab92512 at 1/1000 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    Anti-PAX5 antibody [EPR3729] (ab92512) 使用論文

    This product has been referenced in:
    • Deng J  et al. Applicability of the methylated CpG sites of paired box 5 (PAX5) promoter for prediction the prognosis of gastric cancer. Oncotarget 5:7420-30 (2014). WB ; Human . Read more (PubMed: 25277182) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab92512.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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