製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PAX5
  • アプリケーション
    適用あり: IP, WB, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide from C-terminus of human Pax5 protein (the amino acid sequence is considered to be commercially sensitive).

  • ポジティブ・コントロール
    • Tonsil

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP
WB
IHC-FoFr
  • 追加情報
    Immunohistology (Formalin/paraffin): 1/50 for 30 min at RT.
    Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    WB: Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
    • 関連疾患
      A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
      Leukemia, acute lymphoblastic, 3
    • 配列類似性
      Contains 1 paired domain.
    • 発生段階
      Expressed at early B-cell differentiation, in the developing CNS and in adult testis.
    • 翻訳後修飾
      O-glycosylated.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • B cell lineage specific activator antibody
      • B cell lineage specific activator protein antibody
      • B cell specific activator protein antibody
      • B cell specific transcription factor antibody
      • B-cell-specific transcription factor antibody
      • BSAP antibody
      • EBB-1 antibody
      • KLP antibody
      • Paired box 5 antibody
      • Paired box gene 5 (B cell lineage specific activator protein) antibody
      • Paired box gene 5 (B cell lineage specific activator) antibody
      • Paired box gene 5 antibody
      • Paired box homeotic gene 5 antibody
      • Paired box protein Pax 5 antibody
      • Paired box protein Pax-5 antibody
      • Paired domain gene 5 antibody
      • PAX 5 antibody
      • PAX5 antibody
      • PAX5_HUMAN antibody
      • Transcription factor PAX 5 antibody
      see all

    Anti-PAX5 antibody 画像

    • PAX5 was immunoprecipitated using 0.5mg Ramos whole cell extract, 5µg of Rabbit polyclonal to PAX5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

      The antibody was incubated under agitation with Protein G beads for 10min, Ramos whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15164.

      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

      Band: 42kDa; PAX5

    • ab15164 staining PAX5 in human tonsil by Immunohistochemistry (FFPE-sections).

    Anti-PAX5 antibody (ab15164) 使用論文

    This product has been referenced in:
    • Furukawa S  et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Dog . Read more (PubMed: 28190929) »
    • Perearnau A  et al. p27Kip1, PCAF and PAX5 cooperate in the transcriptional regulation of specific target genes. Nucleic Acids Res 45:5086-5099 (2017). Read more (PubMed: 28158851) »

    See all 3 Publications for this product

    Product Wall

    Vielen Dank für Ihren Anruf gestern.

    Ich habe für Sie unsere anti Pax5 Antikörper durchgeschaut und muss Ihnen leider mitteilen, dass auch alle polyklonalen mittels Immunogen-Peptiden hergestellt wurden. Diese werden demnach h&...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hematopoietic human cell lines)
    Loading amount
    10 µg
    Specification
    hematopoietic human cell lines
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mrs. Grazia Fazio

    Verified customer

    投稿 Jul 23 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse pre-B lymphocytes)
    Loading amount
    5 µg
    Specification
    mouse pre-B lymphocytes
    Gel Running Conditions
    Reduced Denaturing (15%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mrs. Grazia Fazio

    Verified customer

    投稿 Jul 23 2010

    I can confirm that the immunogen sequence is within this amino acid segment and therefore the antibody will recognise your fragment,

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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