製品の概要

  • 製品名Anti-PAX1 antibody
    PAX1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PAX1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (SISRILRNKI GSLAQPGPYE ASKQPPSQPT LPYNHIYQYP YPSPVSPTGA) of Human PAX1 (NP_006183).

  • ポジティブ・コントロール
    • 721_B cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

ターゲット情報

Anti-PAX1 antibody 画像

  • Anti-PAX1 antibody (ab83312) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

Anti-PAX1 antibody (ab83312) 使用論文

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