製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PATL1
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 425-475 of Human PATL1 (NP_689929.2).

  • ポジティブ・コントロール
    • HeLa, 293T and Jurkat whole cell lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 1 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 機能
    RNA-binding protein involved in deadenylation-dependent decapping of mRNAs, leading to the degradation of mRNAs. Acts as a scaffold protein that connects deadenylation and decapping machinery. Required for cytoplasmic mRNA processing body (P-body) assembly. In case of infection, required for translation and replication of hepatitis C virus (HCV).
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the PAT1 family.
  • ドメイン
    The region A, also named N-term, mediates the interaction with DDX6/RCK and is required for cytoplasmic mRNA processing body assembly.
    The region C, also named Pat-C, is required for RNA-binding and mediates the binding with the Lsm-containing SMN-Sm protein complex and the decapping machinery. It folds into an alpha-alpha superhelix, exposing conserved and basic residues on one side of the domain.
  • 細胞内局在
    Cytoplasm > P-body.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hPat1b antibody
    • OK/KNS-cl.5 antibody
    • PAT1-like protein 1 antibody
    • Pat1b antibody
    • PATL1 antibody
    • PATL1_HUMAN antibody
    • protein associated with topoisomerase II homolog 1 antibody
    • Protein PAT1 homolog 1 antibody
    • Protein PAT1 homolog b antibody
    see all

画像

  • All lanes : Anti-PATL1 antibody (ab124257) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 1 kDa


    Exposure time : 3 minutes
  • ab124257 at 1 µg/ml staining PATL1 in HeLa whole cell lysate immunoprecipitated using ab124257 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab124257.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録