製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PATJ
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 540-589 (EVMVATLDTQ IADDAELQKY SKLLPIHTLR LGVEVDSFDG HHYISSIVSG) of Human PATJ (NP_795352).

  • ポジティブ・コントロール
    • 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 196 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性
    PATJ contains multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. PATJ localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors.
  • 細胞内局在
    Membrane. Cell junction; tight junction. Apical cell membrane. Note: Localized in the paranodal region of myelinating Schwann cells. Membrane-associated. Localizes to tight junctions in epithelial cells. Also found at the apical plasma membrane.
  • 参照データベース
  • 別名
    • Channel interacting PDZ domain protein antibody
    • Cipp antibody
    • FLJ26982 antibody
    • hINADL antibody
    • Inactivation no after potential D like protein antibody
    • INAD like antibody
    • InaD like Drosophila antibody
    • INAD like protein antibody
    • INADL antibody
    • INADL protein antibody
    • PALS 1 associated tight junction protein antibody
    • PALS1 associated tight junction protein antibody
    • PATJ antibody
    • PDZ domain protein antibody
    • PDZ domain protein Drosophila inaD like antibody
    • Post synaptic density 95 / discs large / zonula occludens 1 domain protein antibody
    • Protein associated to tight junctions antibody
    see all

画像

  • Anti-PATJ antibody (ab84932) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 196 kDa
    Observed band size: >168 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 120 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration: 6-18%

プロトコール

参考文献

ab84932 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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