製品の概要

  • 製品名
    Anti-Patched / PTCH1 antibody
    Patched / PTCH1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Patched / PTCH1
  • 特異性
    ab53715 detects endogenous levels of total Patched/PTCH1 protein. In WB, in addition to the expected band at 150 kDa an extra band at 75 kDa is often detected. Both bands can be blocked with the immunogen peptide. In IHC, cytoplasmic and membrane staining is frequently observed. These results are similar to what we see with other antibodies against this target.
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human Patched/ PTCH1 (N terminal). Immunogen is in the range of aa 1-50.
    Database link: Q13635

  • ポジティブ・コントロール
    • IHC-P: Human brain tissue WB: Mouse muscle membrane lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 161 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.
  • 組織特異性
    In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin.
  • 関連疾患
    Defects in PTCH1 are probably the cause of basal cell nevus syndrome (BCNS) [MIM:109400]; also known as Gorlin syndrome or Gorlin-Goltz syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas (NBCCS) and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas (BCC), fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 is also mutated in squamous cell carcinoma (SCC). Could also be associated with large body size observed in BCNS patients.
    Defects in PTCH1 are a cause of sporadic basal cell carcinoma (BCC) [MIM:605462].
    Defects in PTCH1 are the cause of holoprosencephaly type 7 (HPE7) [MIM:610828]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.
  • 配列類似性
    Belongs to the patched family.
    Contains 1 SSD (sterol-sensing) domain.
  • 発生段階
    In the embryo, found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud.
  • 翻訳後修飾
    Glycosylation is necessary for SHH binding.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A230106A15Rik antibody
    • BCNS antibody
    • FLJ26746 antibody
    • FLJ42602 antibody
    • Holoprosencephaly 7 antibody
    • HPE7 antibody
    • mes antibody
    • NBCCS antibody
    • OTTHUMP00000021709 antibody
    • OTTHUMP00000021710 antibody
    • Patched antibody
    • Patched (Drosophila) homolog antibody
    • Patched 1 antibody
    • Patched homolog (Drosophila) antibody
    • Patched homolog 1 (Drosophila) antibody
    • Patched homolog 1 antibody
    • Patched protein homolog 1 antibody
    • Protein patched homolog 1 antibody
    • PTC antibody
    • PTC1 antibody
    • PTC1_HUMAN antibody
    • PTCH antibody
    • PTCH protein +12b antibody
    • PTCH protein +4' antibody
    • PTCH protein -10 antibody
    • PTCH protein antibody
    • ptch1 antibody
    • PTCH1 protein antibody
    • PTCH11 antibody
    • Ptch2 antibody
    see all

Anti-Patched / PTCH1 antibody 画像

  • All lanes : Anti-Patched / PTCH1 antibody (ab53715) at 1/500 dilution

    Lane 1 : Extracts from mouse muscle cells, minus immunising peptide
    Lane 2 : Extracts from mouse muscle cells, plus immunising peptide


    Predicted band size : 161 kDa
  • ab53715, at a 1/50 dilution, staining Patched / PTCH in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.ab53715, at a 1/50 dilution, staining Patched / PTCH in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.

Anti-Patched / PTCH1 antibody (ab53715) 使用論文

This product has been referenced in:
  • Celebi AR  et al. Evaluation of the 'Hedgehog' signaling pathways in squamous and basal cell carcinomas of the eyelids and conjunctiva. Oncol Lett 12:467-472 (2016). Read more (PubMed: 27347166) »
  • Pan YB  et al. Sonic hedgehog through Gli2 and Gli3 is required for the proper development of placental labyrinth. Cell Death Dis 6:e1653 (2015). IHC . Read more (PubMed: 25695606) »

See all 8 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate and 10 mM citric acid
Permeabilization
No
Specification
placenta
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 18 2015

Application
Western blot
Sample
Mouse Cell lysate - whole cell (ATDC5)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
70 µg
Treatment
Dicoction for 48 h
Specification
ATDC5
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 18 2015

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (4-15%)
Sample
Human Cell lysate - whole cell (fibroblasts)
Specification
fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Mar 28 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (MKN45)
Specification
MKN45
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount
50 µg
Specification
Mouse ES Cells
Gel Running Conditions
Reduced Denaturing (7% PAGE)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. Utkarsh Kapoor

Verified customer

投稿 Dec 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (chondrocytes)
Loading amount
30 µg
Specification
chondrocytes
Gel Running Conditions
Reduced Denaturing (Bis-Tris 4-20%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Dec 10 2012

Thank you for your enquiry and your interest in our products. The production and the testing conditions of these two antibodies (ab53715 and ab55629) are different.

It is important to mention that ab55629 has only been tested in WB against t...

Read More

Thank you for your enquiry. Both the antibodies ab27529 and ab53715 have been tested in mouse tissue in IHC-P so should be suitable for your use. The datasheet for ab27529 indeed mentions an incubation time of 10 minutes, as this is the originator...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録