Anti-Patched / PTCH1 抗体 (ab109096)

製品の概要

  • 製品名
    Anti-Patched / PTCH1 antibody
    Patched / PTCH1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Patched / PTCH1
  • 由来種
    Goat
  • 特異性
    ab109096 is expected to recognise all reported isoforms. Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1 encode the same isoform.
  • アプリケーション
    適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chicken, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to Human Patched/ PTCH1 aa 1431-1442 (C terminal).
    Sequence:

    ELQDVECEERPR


    Database link: Q13635

  • ポジティブ・コントロール
    • Human Colon tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 3 - 6 µg/ml. Steamed antigen retrieval with citrate buffer pH 6.

ターゲット情報

  • 機能
    Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.
  • 組織特異性
    In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin.
  • 関連疾患
    Defects in PTCH1 are probably the cause of basal cell nevus syndrome (BCNS) [MIM:109400]; also known as Gorlin syndrome or Gorlin-Goltz syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas (NBCCS) and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas (BCC), fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 is also mutated in squamous cell carcinoma (SCC). Could also be associated with large body size observed in BCNS patients.
    Defects in PTCH1 are a cause of sporadic basal cell carcinoma (BCC) [MIM:605462].
    Defects in PTCH1 are the cause of holoprosencephaly type 7 (HPE7) [MIM:610828]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.
  • 配列類似性
    Belongs to the patched family.
    Contains 1 SSD (sterol-sensing) domain.
  • 発生段階
    In the embryo, found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud.
  • 翻訳後修飾
    Glycosylation is necessary for SHH binding.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A230106A15Rik antibody
    • BCNS antibody
    • FLJ26746 antibody
    • FLJ42602 antibody
    • Holoprosencephaly 7 antibody
    • HPE7 antibody
    • mes antibody
    • NBCCS antibody
    • OTTHUMP00000021709 antibody
    • OTTHUMP00000021710 antibody
    • Patched antibody
    • Patched (Drosophila) homolog antibody
    • Patched 1 antibody
    • Patched homolog (Drosophila) antibody
    • Patched homolog 1 (Drosophila) antibody
    • Patched homolog 1 antibody
    • Patched protein homolog 1 antibody
    • Protein patched homolog 1 antibody
    • PTC antibody
    • PTC1 antibody
    • PTC1_HUMAN antibody
    • PTCH antibody
    • PTCH protein +12b antibody
    • PTCH protein +4' antibody
    • PTCH protein -10 antibody
    • PTCH protein antibody
    • ptch1 antibody
    • PTCH1 protein antibody
    • PTCH11 antibody
    • Ptch2 antibody
    see all

画像

  • Immunohistochemical analysis of Human breast tissue labeling Patched/PTCH1. Steamed antigen retrieval with citrate buffer pH
  • Immunofluorescence analysis of paraformaldehyde fixed NIH/3T3 cells staining Patched/PTCH1. Cells were permeabilized with 0.15% Triton. Samples were incubated with primary anitbody for 1 hour at 10µg/ml. An Alexa Fluor® 488 was used as the secondary antibody. DAPI was used as a nuclear counterstain. Unimmunized goat IgG (10µg/ml) was used as the negative control.
  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cells staining Patched/PTCH1. Cells were permeabilized with 0.15% Triton. Samples were incubated with primary anitbody for 1 hour at 5µg/ml. An Alexa Fluor® 488 was used as the secondary antibody at 1µg/ml. DAPI was used as a nuclear counterstain.
  • ab109096 at 3.8µg/ml staining Patched / PTCH1 in Human Colon by Immunohistochemistry, Paraffin-embedded tissue.
    Note: Textured nuclear staining of the absorptive cells.

プロトコール

参考文献

ab109096 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (adrenal gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: tris
Permeabilization
No
Specification
adrenal gland
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Dr. Michael Thomas

Verified customer

投稿 Dec 06 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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