Anti-Parvalbumin 抗体 (ab32895)

製品の概要

  • 製品名Anti-Parvalbumin antibody
    Parvalbumin 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Parvalbumin
  • アプリケーション適用あり: WB, ELISA, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Xenopus laevis, Macaque Monkey
  • 免疫原

    Synthetic peptide:

    GVDEFSTLVAES

    , corresponding to C terminal amino acids 98-109 of Human Parvalbumin

  • ポジティブ・コントロール
    • Human Brain lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab32895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
ELISA 1/32000.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

Anti-Parvalbumin antibody 画像

  • Anti-Parvalbumin antibody (ab32895) at 0.1 µg/ml + Human Brain lysate at 35 µg

    Predicted band size : 12 kDa
    Observed band size : 12 kDa
  • Anti-Parvalbumin antibody (ab32895) at 0.2 µg/ml + whole murine brain tissue lysate at 20 µg

    Secondary
    Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/8000 dilution
    Developed using the ECL technique

    Predicted band size : 12 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

Anti-Parvalbumin antibody (ab32895) 使用論文

ab32895 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 27°C
Sample Mouse Tissue sections (frozen brain section)
Specification frozen brain section
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 07 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain (hippocampus))
Specification Brain (hippocampus)
Fixative Paraformaldehyde
Permeabilization No
Username

Abcam user community

Verified customer

投稿 Dec 19 2012

Merci de nous avoir contactés. L'anti-Parvalbumine d'Abcam, référence ab32895, est un polyclonal de chèvre alors que l'anticorps d'Alpha Diagnostic est un monoclonal de souris. J'espère que ces informations vous seront utiles. N'hésitez pas à nou...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (brain)
Loading amount 20 µg
Specification brain
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"