The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
Poly [ADP-ribose] polymerase using NAD(+) as a substrate to transfer ADP-ribose onto glutamic acid residues of a protein acceptor; repeated rounds of ADP-ribosylation leads to the formation of poly(ADPribose) chains on the protein, thereby altering the function of the target protein. May play a role in the adaptative response to chemical exposure (TCDD) and thereby mediates certain effects of the chemicals.
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human hepatocarcinoma tissue using ab80068 at a 1/10 dilution, with a peroxidase-conjugated secondary antibody followed by DAB staining.