製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PARP2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Human PARP2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Human brain; Human pancreas; Human testis.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).

ターゲット情報

  • 機能
    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
  • 組織特異性
    Widely expressed, mainly in actively dividing tissues. The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine, prostate and thymus.
  • 配列類似性
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
  • 翻訳後修飾
    Poly-ADP-ribosylated by PARP1.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADP ribosyltransferase like 2 antibody
    • ADP-ribosyltransferase diphtheria toxin-like 2 antibody
    • ADPRT 2 antibody
    • ADPRT-2 antibody
    • ADPRT2 antibody
    • ADPRTL 2 antibody
    • ADPRTL 3 antibody
    • ADPRTL2 antibody
    • ADPRTL3 antibody
    • ARTD2 antibody
    • hPARP 2 antibody
    • hPARP-2 antibody
    • hPARP2 antibody
    • NAD(+) ADP ribosyltransferase 2 antibody
    • NAD(+) ADP-ribosyltransferase 2 antibody
    • pADPRT 2 antibody
    • pADPRT-2 antibody
    • pADPRT2 antibody
    • PARP 2 antibody
    • PARP-2 antibody
    • PARP2 antibody
    • PARP2_HUMAN antibody
    • Poly (ADP ribose) polymerase family member 2 antibody
    • Poly (ADP ribosyl) transferase like 2 antibody
    • poly (ADP-ribose) polymerase 2 antibody
    • Poly [ADP ribose] synthetase 2 antibody
    • Poly [ADP-ribose] polymerase 2 antibody
    • Poly(ADP ribose) synthetase antibody
    • Poly[ADP-ribose] synthase 2 antibody
    see all

画像

  • All lanes : Anti-PARP2 antibody (ab93841) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 3 : Human testis tissue lysate - total protein (ab30257)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 66 kDa
    Observed band size : 66 kDa
    Additional bands at : 57 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

プロトコール

参考文献

This product has been referenced in:
  • Peña-Hernández R  et al. Genome-wide targeting of the epigenetic regulatory protein CTCF to gene promoters by the transcription factor TFII-I. Proc Natl Acad Sci U S A 112:E677-86 (2015). WB . Read more (PubMed: 25646466) »

See 1 Publication for this product

レビューと Q&A

Thank you very much for your interest in ab93841.

Unfortunately, the immunogen has only about 66% of identity with the arabidopsis sequence. The possibility of crossreaction is therefore low. Please see here for the alignment yourself:
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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