製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PARP10
  • アプリケーション
    適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to a region between residues 300 and 350 of Human PARP10 (NP_116178.1)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab70800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/2500. Detects a band of approximately 123 kDa (predicted molecular weight: 123 kDa).
IP Use at 1-4 µg/mg of lysate.

ターゲット情報

  • 関連性
    Poly (ADP ribose) polymerases (PARPs), such as PARP10, regulate gene transcription by altering chromatin organization by adding ADP ribose to histones. PARPs can also function as transcriptional cofactors
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • ADP ribosyltransferase diphtheria toxin like 10 antibody
    • ARTD10 antibody
    • PARP 10 antibody
    • Poly (ADP ribose) polymerase family member 10 antibody
    • Poly [ADP ribose] polymerase 10 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PARP10 with ab70800 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PARP10 antibody (ab70800) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 123 kDa
    Observed band size : 123 kDa
    Additional bands at : 60 kDa,70 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • 1mg of whole cell lysate from HeLa cells was immunoprecipitated using ab70800 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of immunoprecipitate was loaded per lane, and probed with ab70800 at 1ug/ml. Detection: chemiluminescence with exposure time of 30 seconds.

参考文献

ab70800 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab70800.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録