The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/500 - 1/2500. Detects a band of approximately 123 kDa (predicted molecular weight: 123 kDa).
Use at 1-4 µg/mg of lysate.
Poly (ADP ribose) polymerases (PARPs), such as PARP10, regulate gene transcription by altering chromatin organization by adding ADP ribose to histones. PARPs can also function as transcriptional cofactors
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PARP10 with ab70800 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - PARP10 antibody (ab70800)
All lanes : Anti-PARP10 antibody (ab70800) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 123 kDa Observed band size: 123 kDa Additional bands at: 60 kDa, 70 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Immunoprecipitation - PARP10 antibody (ab70800)
1mg of whole cell lysate from HeLa cells was immunoprecipitated using ab70800 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of immunoprecipitate was loaded per lane, and probed with ab70800 at 1ug/ml. Detection: chemiluminescence with exposure time of 30 seconds.