Anti-PARP1 抗体 (ab194586)
Key features and details
- Rabbit polyclonal to PARP1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PARP1 antibody
PARP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PARP1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human PARP1 aa 150-250. The exact sequence is proprietary.
Database link: P09874 -
ポジティブ・コントロール
- WB: Extracts from A549, Jurkat, HeLa, K562, Raji, HepG2 and Y79 cell lines. Mouse testis lysate. ICC/IF: NIH/3T3 and U-2 OS cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab194586の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/20 - 1/50.
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WB | (1) |
1/1000 - 1/2000. Predicted molecular weight: 113 kDa.
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ICC/IF |
1/50 - 1/100.
|
特記事項 |
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IHC-P
1/20 - 1/50. |
WB
1/1000 - 1/2000. Predicted molecular weight: 113 kDa. |
ICC/IF
1/50 - 1/100. |
ターゲット情報
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機能
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. -
配列類似性
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers. -
翻訳後修飾
Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 142 Human
- Entrez Gene: 11545 Mouse
- Entrez Gene: 25591 Rat
- Omim: 173870 Human
- SwissProt: P09874 Human
- SwissProt: P11103 Mouse
- SwissProt: P27008 Rat
- Unigene: 177766 Human
see all -
別名
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
- ADP ribosyltransferase antibody
- ADP ribosyltransferase diphtheria toxin like 1 antibody
see all
画像
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All lanes : Anti-PARP1 antibody (ab194586) at 1/1000 dilution
Lane 1 : Extract from A549 (Human lung carcinoma cell line) cell line
Lane 2 : Extract from Jurkat (Human T cell leukemia cell line from peripheral blood) cell line
Lane 3 : Extract from Hela (Human epithelial cell line from cervix adenocarcinoma) cell line
Lane 4 : Extract from K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell line
Lane 5 : Extract from Raji (Human Burkitt's lymphoma cell line) cell line
Lane 6 : Extract from HepG2 (Human liver hepatocellular carcinoma cell line) cell line
Lane 7 : Extract from Y79 (Human retinoblastoma cell line) cell line
Predicted band size: 113 kDa -
U-2 OS (Human bone osteosarcoma epithelial cell line) cells stained for PARP1 using ab194586 at a dilution of 1/100 in ICC/IF. Blue: DAPI for nuclear staining.
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Immunohistochemistry of Human breast cancer tissue staining PARP1 with ab194586
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Immunohistochemistry of Human appendix tissue staining PARP1 with ab194586
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Immunohistochemistry of rat testis tissue staining PARP1 with ab194586
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NIH-3T3 (Mouse embryo fibroblast cell line) cells stained for PARP1 using ab194586 at a dilution of 1/100 in ICC/IF. Blue: DAPI for nuclear staining.
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Anti-PARP1 antibody (ab194586) at 1/1000 dilution + Mouse testis lysate at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 113 kDa
Exposure time: 90 secondsBlocking buffer: 3% non-fat dry milk in TBST.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab194586 は 15 報の論文で使用されています。
- Zhang L et al. Positive Feedback Regulation of Poly(ADP-ribose) Polymerase 1 and the DNA-PK Catalytic Subunit Affects the Sensitivity of Nasopharyngeal Carcinoma to Etoposide. ACS Omega 7:2571-2582 (2022). PubMed: 35097256
- Zhang H et al. [18F]-Labeled PARP-1 PET imaging of PSMA targeted alpha particle radiotherapy response. Sci Rep 12:13034 (2022). PubMed: 35906379
- Biswas S et al. PEITC: A resounding molecule averts metastasis in breast cancer cells in vitro by regulating PKCδ/Aurora A interplay. Heliyon 8:e11656 (2022). PubMed: 36458309
- D'Onofrio N et al. Colorectal Cancer Apoptosis Induced by Dietary δ-Valerobetaine Involves PINK1/Parkin Dependent-Mitophagy and SIRT3. Int J Mol Sci 22:N/A (2021). PubMed: 34360883
- Xu L et al. Quantitative global proteome and phosphorylome analyses reveal potential biomarkers in kidney cancer. Oncol Rep 46:N/A (2021). PubMed: 34528699