製品の概要

  • 製品名Anti-PARP antibody [Y17]
    PARP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y17] to PARP
  • 特異性This antibody is specific to PARP. It should recognize the intact form (116kDa) and the p25 cleaved form of PARP.
  • アプリケーション適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chinese Hamster
  • 免疫原

    corresponding to Human PARP.

  • ポジティブ・コントロール
    • WB: Jurkat and Camptothecin cell lysate.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab32378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 113 kDa).
IP 1/80.
  • 追加情報Is unsuitable for Flow Cyt,ICC or IHC.
  • ターゲット情報

    • 機能Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
    • 配列類似性Contains 1 BRCT domain.
      Contains 1 PARP alpha-helical domain.
      Contains 1 PARP catalytic domain.
      Contains 2 PARP-type zinc fingers.
    • 翻訳後修飾Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
      Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
      S-nitrosylated, leading to inhibit transcription regulation activity.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
      • ADP ribosyltransferase diphtheria toxin like 1 antibody
      • ADP ribosyltransferase NAD(+) antibody
      • ADPRT 1 antibody
      • ADPRT antibody
      • ADPRT1 antibody
      • ARTD1 antibody
      • msPARP antibody
      • NAD(+) ADP ribosyltransferase 1 antibody
      • NAD(+) ADP-ribosyltransferase 1 antibody
      • pADPRT 1 antibody
      • pADPRT1 antibody
      • PARP 1 antibody
      • PARP antibody
      • PARP-1 antibody
      • PARP1 antibody
      • PARP1_HUMAN antibody
      • Poly (ADP ribose) polymerase 1 antibody
      • poly (ADP ribose) polymerase family, member 1 antibody
      • Poly [ADP-ribose] polymerase 1 antibody
      • Poly(ADP ribose) polymerase antibody
      • poly(ADP ribose) synthetase antibody
      • poly(ADP ribosyl)transferase antibody
      • Poly[ADP ribose] synthetase 1 antibody
      • Poly[ADP-ribose] synthase 1 antibody
      • PPOL antibody
      see all

    Anti-PARP antibody [Y17] 画像



    • Predicted band size : 113 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: PARP1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: MCF7 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab32378 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32378 was shown to specifically react with PARP1 when PARP1 knockout samples were used. Wild-type and PARP1 knockout samples were subjected to SDS-PAGE. Ab32378 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-PARP antibody [Y17] (ab32378) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate.
      Lane 2 : Jurkat cell lysate and Camptothecin cell lysate.


      Predicted band size : 113 kDa
      Observed band size : 115 kDa (why is the actual band size different from the predicted?)

    Anti-PARP antibody [Y17] (ab32378) 使用論文

    ab32378 has not yet been referenced specifically in any publications.

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