製品の概要

  • 製品名Anti-PARN antibody
    PARN 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PARN
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human PARN.

    (Peptide available as ab28513.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HeLa, Jurkat and A431 whole cell lysates

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab27778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
IHC-P
  • 追加情報ICC/IF: 1/100 (please see abreview).
    IHC-P: 1/25. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 73 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.
    • 組織特異性Ubiquitous.
    • 配列類似性Belongs to the CAF1 family.
      Contains 1 R3H domain.
    • 細胞内局在Nucleus. Cytoplasm. Nucleus > nucleolus. Some nuclear fraction is nucleolar.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DAN antibody
      • Deadenylating nuclease antibody
      • Deadenylation nuclease antibody
      • PARN antibody
      • PARN_HUMAN antibody
      • Poly A specific ribonuclease antibody
      • Poly(A) specific ribonuclease antibody
      • Poly(A)-specific ribonuclease PARN antibody
      • Polyadenylate specific ribonuclease antibody
      • Polyadenylate-specific ribonuclease antibody
      see all

    Anti-PARN antibody 画像

    • All lanes : Anti-PARN antibody (ab27778) at 1 µg/ml

      Lane 1 : HeLa Whole Cell Lysate
      Lane 2 : Jurkat whole cell lysate (ab7899)
      Lane 3 : A431 whole cell lysate (ab7909)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 73 kDa
    • ab27778, at 1/100 dilution, staining human PARN in HeLa cells.

      See Abreview

    • Image courtesy of Human Protein Atlas

      ab27778 staining PARN in human small intestine. Paraffin embedded human small intestine tissue was incubated with ab27778 (1/25 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

      ab27778 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

      Further results for this antibody can be found at www.proteinatlas.org

    Anti-PARN antibody (ab27778) 使用論文

    ab27778 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    投稿 Dec 18 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"