製品の概要

  • 製品名Anti-PARN antibody
    PARN 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PARN
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 589-639 of Human PARN (NP_002573.1).

  • ポジティブ・コントロール
    • HeLa, Jurkat and 293T whole cell lysates

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab125185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 機能3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the CAF1 family.
    Contains 1 R3H domain.
  • 細胞内局在Nucleus. Cytoplasm. Nucleus > nucleolus. Some nuclear fraction is nucleolar.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DAN antibody
    • Deadenylating nuclease antibody
    • Deadenylation nuclease antibody
    • PARN antibody
    • PARN_HUMAN antibody
    • Poly A specific ribonuclease antibody
    • Poly(A) specific ribonuclease antibody
    • Poly(A)-specific ribonuclease PARN antibody
    • Polyadenylate specific ribonuclease antibody
    • Polyadenylate-specific ribonuclease antibody
    see all

Anti-PARN antibody 画像

  • All lanes : Anti-PARN antibody (ab125185) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 73 kDa


    Exposure time : 3 minutes
  • ab125185, at 6 µg/mg lysate, detecting PARN in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
    For the subsequent Western blot, ab125185 was used at 1 µg/ml.
    The blot was developed using the ECL technique, with an exposure time of 3 seconds.

Anti-PARN antibody (ab125185) 使用論文

ab125185 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"