製品の概要

  • 製品名Anti-PARL antibody
    PARL 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PARL
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human PARL.

    (Peptide available as ab45230.)

  • ポジティブ・コントロール
    • ab45231 gave a positive result in the following whole cell lysates: Hela (data not shown), Jurkat, A431, HEK293 (data not shown), MCF7, U20S (data not shown). IHC-P: human colon adenocarcinoma FFPE tissue sections This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 42 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptoptic signals (By similarity). Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain.
  • 配列類似性Belongs to the peptidase S54 family.
  • 翻訳後修飾P-beta is proteolytically processed (beta-cleavage) in a PARL-dependent manner. The cleavage is inhibited when residues Ser-65, Thr-69 and Ser-70 are all phosphorylated.
  • 細胞内局在Mitochondrion inner membrane and Nucleus. Translocated into the nucleus by an unknown mechanism.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Mitochondrial intramembrane cleaving protease PARL antibody
    • Mitochondrial intramembrane-cleaving protease PARL antibody
    • P-beta antibody
    • parl antibody
    • PARL_HUMAN antibody
    • Pbeta antibody
    • presenilin associated rhomboid-like antibody
    • Presenilins associated rhomboid-like protein antibody
    • PRO2207 antibody
    • PSARL antibody
    • PSARL1 antibody
    • PSENIP2 antibody
    • RHBDS1 antibody
    • Rhomboid 7 homolog 1 antibody
    see all

Anti-PARL antibody 画像

  • ICC/IF image of ab45231 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab45231 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-PARL antibody (ab45231) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa,51 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    PARL is a mitochondrial protein which is constitutively cleaved, leaving a protein of 36.5 kDa (Sík A et al, J Biol Chem, 2004 Apr 9;279(15):15323-9 (PMID 14732705)).  ab45231 detects a band at 36 kDa (cleaved form).  However, Abcam's data suggests that ab45231 does not detect the full PARL protein (42 kDa).

  • IHC image of PARL staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45231, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PARL antibody (ab45231) 使用論文

ab45231 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample Mouse Cell (MEF)
Specification MEF
Permeabilization Yes - 1% TRITON-X-100
Fixative Formaldehyde
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投稿 Jul 03 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample Rat Cell (h9c2)
Specification h9c2
Permeabilization Yes - 1% TRITON-X-100
Fixative Formaldehyde
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投稿 Jul 03 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Sample Human Cell (HEK293)
Specification HEK293
Permeabilization Yes - 1% TRITON-X-100
Fixative Formaldehyde
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投稿 Jul 03 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (MEF)
Specification MEF
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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投稿 Jan 02 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (h9c2)
Specification h9c2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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投稿 Jan 02 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (hek293)
Specification hek293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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投稿 Jan 02 2014

Abreviews
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - other (Brain mitochondria and total brain lysate)
Specification Brain mitochondria and total brain lysate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Nov 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (Mitochondria isolated from HEK293T cells)
Loading amount 24 µg
Specification Mitochondria isolated from HEK293T cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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Mr. Andrew Greene

Verified customer

投稿 May 23 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
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Dr. Sophie Pezet

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投稿 Oct 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"