Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human PARL.
(Peptide available as ab45230.)
Our Abpromise guarantee covers the use of ab45231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 42 kDa). | |
IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
ICC/IF | Use a concentration of 5 µg/ml. |
ICC/IF image of ab45231 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab45231 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
PARL is a mitochondrial protein which is constitutively cleaved, leaving a protein of 36.5 kDa (Sík A et al, J Biol Chem, 2004 Apr 9;279(15):15323-9 (PMID 14732705)). ab45231 detects a band at 36 kDa (cleaved form). However, Abcam's data suggests that ab45231 does not detect the full PARL protein (42 kDa).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"