Anti-PARK7/DJ1 抗体 [malphaDJ-1/E2.19] (ab11251)
製品の概要
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製品名Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19]
PARK7/DJ1 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [malphaDJ-1/E2.19] to PARK7/DJ1
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由来種Mouse
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特異性This clone has been shown to specifically recognise a fusion protein of PARK7/DJ1. It also recognises a FLAG-tagged PARK7/DJ1 expressed in eukaryotic cells. A single band is seen when Western blotting in optimised conditions with this clone. However, overloading the gel or using low dilutions can cause other bands to appear.
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アプリケーション適用あり: Flow Cyt, IHC-Fr, ICC/IF, WB, ICC, IHC-FoFrmore details
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種交差性交差種: Human, Zebrafish
非交差種: Mouse -
免疫原
Recombinant full length protein (Human).
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ポジティブ･コントロール
- WB: HeLa whole cell lysate. Flow Cytometry: HepG2 cells.
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特記事項
This antibody clone is manufactured by Abcam.
This monoclonal antibody to DJ-1 has been knockout validated in Western blot. The expected band for DJ-1 was observed in wild type cells and the band was not seen in knockout cells.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度Concentrated Culture Supernatant
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ポリ/モノモノクローナル
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クローン名malphaDJ-1/E2.19
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アイソタイプIgM
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研究分野
関連製品
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Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
- Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772)
- Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
- Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
- Goat Anti-Mouse IgM H&L (ab9167)
- Rabbit Anti-Mouse IgM mu chain (ab9175)
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Isotype control
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab11251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
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| Flow Cyt | 1/100. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
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| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | 1/500. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 20 kDa. | |
| ICC | Use at an assay dependent concentration. | |
| IHC-FoFr | 1/1000. |
ターゲット情報
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機能Protects cells against oxidative stress and cell death. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. May act as an atypical peroxiredoxin-like peroxidase that scavenges hydrogen peroxide. Following removal of a C-terminal peptide, displays protease activity and enhanced cytoprotective action against oxidative stress-induced apoptosis. Stabilizes NFE2L2 by preventing its association with KEAP1 and its subsequent ubiquitination. Binds to OTUD7B and inhibits its deubiquitinating activity. Enhances RELA nuclear translocation. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Required for correct mitochondrial morphology and function and for autophagy of dysfunctional mitochondria. Regulates astrocyte inflammatory responses. Acts as a positive regulator of androgen receptor-dependent transcription. Prevents aggregation of SNCA. Plays a role in fertilization. Has no proteolytic activity. Has cell-growth promoting activity and transforming activity. May function as a redox-sensitive chaperone.
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組織特異性Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain. Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa.
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関連疾患Defects in PARK7 are the cause of Parkinson disease type 7 (PARK7) [MIM:606324]. A neurodegenerative disorder characterized by resting tremor, postural tremor, bradykinesia, muscular rigidity, anxiety and psychotic episodes. PARK7 has onset before 40 years, slow progression and initial good response to levodopa. Some patients may show traits reminiscent of amyotrophic lateral sclerosis-parkinsonism/dementia complex (Guam disease).
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配列類似性Belongs to the peptidase C56 family.
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翻訳後修飾Sumoylated on Lys-130 by PIAS2 or PIAS4; which is enhanced after ultraviolet irradiation and essential for cell-growth promoting activity and transforming activity.
Cys-106 is easily oxidized to sulfinic acid.
Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. -
細胞内局在Cytoplasm. Nucleus. Mitochondrion. Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage. Detected in tau inclusions in brains from neurodegenerative disease patients.
- Information by UniProt
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参照データベース
- Entrez Gene: 11315 Human
- Entrez Gene: 449674 Zebrafish
- Omim: 602533 Human
- SwissProt: Q99497 Human
- SwissProt: Q5XJ36 Zebrafish
- Unigene: 419640 Human
- Unigene: 85181 Zebrafish
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別名
- CAP1 antibody
- DJ-1 antibody
- DJ1 antibody
see all
画像
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![Western blot - PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)](http://a.abcam.com/ps/products/11/ab11251/Images/ab11251_2.jpg)
Western blot - PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)Western blot using ab11251 at 1/500 on HeLa whole cell lysate (20
Western blot using ab11251 at 1/500 on HeLa whole cell lysate (20µg/lane).µ -
![- PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)](http://a.abcam.com/ps/products/11/ab11251/Images/ab11251_1.jpg)
- PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)Western blot using clone malphaDJ-1/E2.19 and a beta actin antibody as a loading control.
The bottom band is PARK7/DJ1, the top band is beta actin.
Lane 1: 293 cell lysate
Lane 2: MCF-7 cell lysate
Lanes 3-7: various different prostate cell lines
Lane 8: recombinant PARK7/DJ1 (that was used as immunogen for this antibody)
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![Western blot - Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)](http://a.abcam.com/ps/products/11/ab11251/Images/ab11251-261814-ab11251KOWB.jpg)
Western blot - Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PARK7/DJ1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab11251 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.ab11251 was shown to specifically react with PARK7/DJ1 in wild-type HAP1 cells. No band was observed when knockout samples were used. Wild-type and PARK7/DJ1 knockout samples were subjected to SDS-PAGE. ab11251 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)](http://a.abcam.com/ps/products/11/ab11251/Images/ab11251-IF-Im2.jpg)
Immunocytochemistry/ Immunofluorescence - PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)ICC/IF image of ab11251 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab11251, 1:500 dilution) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). -
![Flow Cytometry - Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)](http://a.abcam.com/ps/products/11/ab11251/Images/PARK7DJ1-Primary-antibodies-ab11251-3.jpg)
Flow Cytometry - Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)Overlay histogram showing HepG2 cells stained with ab11251 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11251, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
参考文献
This product has been referenced in:
- Qin LX et al. BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage. Oxid Med Cell Longev 2017:5094934 (2017). Read more (PubMed: 28348719) »
- Principe S et al. Identification of prostate-enriched proteins by in-depth proteomic analyses of expressed prostatic secretions in urine. J Proteome Res 11:2386-96 (2012). WB . Read more (PubMed: 22339264) »
レビューと Q&A
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