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Recombinant full length protein (Human).
Our Abpromise guarantee covers the use of ab11251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/100. ab91545-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 20 kDa.|
|ICC||Use at an assay dependent concentration.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PARK7/DJ1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab11251 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab11251 was shown to recognize PARK7/DJ1 when PARK7/DJ1 knockout samples were used, along with additional cross-reactive bands. Wild-type and PARK7/DJ1 knockout samples were subjected to SDS-PAGE. ab11251 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot using clone malphaDJ-1/E2.19 and a beta actin antibody as a loading control.
The bottom band is PARK7/DJ1, the top band is beta actin.
Lane 1: 293 cell lysate
Lane 2: MCF-7 cell lysate
Lanes 3-7: various different prostate cell lines
Lane 8: recombinant PARK7/DJ1 (that was used as immunogen for this antibody)
Western blot using ab11251 at 1/500 on HeLa whole cell lysate (20