Anti-PAR-3/PARD3 抗体 [EP310Y] (ab40769)
Key features and details
- Rabbit monoclonal [EP310Y] to PAR-3/PARD3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-PAR-3/PARD3 antibody [EP310Y]
PAR-3/PARD3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP310Y] to PAR-3/PARD3 -
由来種
Rabbit -
特異性
This antibody is predicted to detect isoforms 2-7 based on sequence analysis. -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF,IHC or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: LNCap, HeLa, A431, MCF-7
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP310Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40769の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
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特記事項 |
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WB
1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa). |
ターゲット情報
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機能
Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the PAR3 family.
Contains 3 PDZ (DHR) domains. -
ドメイン
Contains a conserved N-terminal oligomerization domain (NTD) that is involved in oligomerization and is essential for proper subapical membrane localization.
The second PDZ domain mediates interaction with membranes containing phosphoinositol lipids. -
翻訳後修飾
Phosphorylated by PRKCZ. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2.
Phosphorylation by STK6/AURKA at Ser-962 is required for the normal establishment of neuronal polarity. -
細胞内局在
Endomembrane system. Cell junction. Cell junction > tight junction. Cell membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. - Information by UniProt
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参照データベース
- Entrez Gene: 56288 Human
- Omim: 606745 Human
- SwissProt: Q8TEW0 Human
- Unigene: 131489 Human
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別名
- agouti signaling protein antibody
- ASIP antibody
- Atypical PKC isotype specific interacting protein antibody
see all
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab40769 は 2 報の論文で使用されています。
- Wu Z et al. lncRNA NEAT1 Downregulation Ameliorates the Myocardial Infarction of Mice by Regulating the miR-582-5p/F2RL2 Axis. Cardiovasc Ther 2022:4481360 (2022). PubMed: 36540097
- Cho JH et al. Identification of the novel substrates for caspase-6 in apoptosis using proteomic approaches. BMB Rep 46:588-93 (2013). PubMed: 24195789