Anti-PARD3 抗体 [EP310Y] (ab40769)


  • 製品名Anti-PARD3 antibody [EP310Y]
    PARD3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP310Y] to PARD3
  • 特異性This antibody is predicted to detect isoforms 2-7 based on sequence analysis.
  • アプリケーション適用あり: WB, Flow Cytmore details
    適用なし: ICC,IHC or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of human PARD3. Does not react with PARD 4 or PARD 1.

  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab40769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for ICC,IHC or IP.
  • ターゲット情報

    • 機能Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.
    • 組織特異性Widely expressed.
    • 配列類似性Belongs to the PAR3 family.
      Contains 3 PDZ (DHR) domains.
    • ドメインContains a conserved N-terminal oligomerization domain (NTD) that is involved in oligomerization and is essential for proper subapical membrane localization.
      The second PDZ domain mediates interaction with membranes containing phosphoinositol lipids.
    • 翻訳後修飾Phosphorylated by PRKCZ. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2.
      Phosphorylation by STK6/AURKA at Ser-962 is required for the normal establishment of neuronal polarity.
    • 細胞内局在Endomembrane system. Cell junction. Cell junction > tight junction. Cell membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
    • Information by UniProt
    • 参照データベース
    • 別名
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    Anti-PARD3 antibody [EP310Y] 画像

    • All lanes : Anti-PARD3 antibody [EP310Y] (ab40769) at 1/500 dilution

      Lane 1 : LNCap
      Lane 2 : HeLa
      Lane 3 : A431
      Lane 4 : MCF-7

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 150 kDa
      Observed band size : 150 kDa

    Anti-PARD3 antibody [EP310Y] (ab40769) 使用論文

    ab40769 has not yet been referenced specifically in any publications.

    Product Wall

    Based on the sequence alignment, I could not find any information which suggested this antibody would detect PAR4 or 1. I hope this information helps and please don't hesitate to contact us for more advice or information in the future.