The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 281 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Cytoplasmic anchor protein in p53-associated protein complex. Regulates the subcellular localization of p53 and subsequent function. Seems to be part of an atypical cullin-RING-based E3 ubiquitin-protein ligase complex. In vitro, complexes of CUL9/PARC with either CUL7 or TP53 contain E3 ubiquitin-protein ligase activity.
Ubiquitously expressed in all tissues with highest expression in testis brain and kidney.
Belongs to the cullin family. Contains 1 DOC domain. Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers.
The IBR domain is required for interaction with UBCH7 and UBCH8.
In vitro, self-ubiquitination in the presence of E1, E2 and ubiquitin. Neddylated.
ab115631 at 5ug/ml staining PARC in Human testis tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.