Anti-Parathyroid Hormone Receptor 1 抗体 (ab75150)

製品の概要

  • 製品名
    Anti-Parathyroid Hormone Receptor 1 antibody
    Parathyroid Hormone Receptor 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Parathyroid Hormone Receptor 1
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human, Lizard
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from internal sequence of human Parathyroid Hormone Receptor 1.

  • ポジティブ・コントロール
    • Human brain tissue. Extracts from Jurkat cells. MCF7 cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 66 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 機能
    Receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
  • 組織特異性
    Expressed in most tissues. Most abundant in kidney, bone and liver.
  • 関連疾患
    Jansen metaphyseal chondrodysplasia
    Chondrodysplasia Blomstrand type
    Enchondromatosis multiple
    Eiken skeletal dysplasia
    Primary failure of tooth eruption
  • 配列類似性
    Belongs to the G-protein coupled receptor 2 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC138426 antibody
    • MGC138452 antibody
    • Parathyroid hormone 1 receptor antibody
    • Parathyroid hormone/parathyroid hormone related peptide receptor antibody
    • Parathyroid hormone/parathyroid hormone related protein receptor antibody
    • Parathyroid hormone/parathyroid hormone-related peptide receptor antibody
    • PTH receptor antibody
    • PTH/PTHr receptor antibody
    • PTH/PTHrP receptor antibody
    • PTH/PTHrP type I receptor antibody
    • PTH1 receptor antibody
    • PTH1R antibody
    • PTH1R_HUMAN antibody
    • PTHR 1 antibody
    • PTHR antibody
    • PTHR1 antibody
    see all

Anti-Parathyroid Hormone Receptor 1 antibody 画像

  • ab75150 staining Parathyroid Hormone Receptor 1 in Lizard regenerated tail (Anolis sagrei) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% serum for 45 minutes at 22°C; antigen retrieval was by heat mediation in sodium citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/200 in 1% horse serum) for 16 hours at 4°C. A Biotin-conjugated horse anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.

    Image is of a regenerated lizard tail cartilage tube, at a proximal region that has begun to ossify. Positive staining was observed in structures resembling embryonic bone collar and early hypertrophic chondrocytes.

    See Abreview

  • Immunohistochemistry analysis of Parathyroid Hormone Receptor 1 in paraffin-embedded human brain tissue using ab75150, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • All lanes : Anti-Parathyroid Hormone Receptor 1 antibody (ab75150) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 66 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 117 kDa,82 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of Parathyroid Hormone Receptor 1 in MCF7 cells using ab75150, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-Parathyroid Hormone Receptor 1 antibody (ab75150) 使用論文

This product has been referenced in:
  • Hu J  et al. Filamin B regulates chondrocyte proliferation and differentiation through Cdk1 signaling. PLoS One 9:e89352 (2014). WB, ICC/IF ; Mouse . Read more (PubMed: 24551245) »
  • Horie M  et al. Intra-articular injection of human mesenchymal stem cells (MSCs) promote rat meniscal regeneration by being activated to express Indian hedgehog that enhances expression of type II collagen. Osteoarthritis Cartilage 20:1197-207 (2012). Read more (PubMed: 22750747) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Kidney, Liver)
Permeabilization
No
Specification
Kidney, Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Daniel Tausan

Verified customer

投稿 Mar 31 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate buffer, pH 6.0
Sample
Lizard Tissue sections (Regenerated lizard tail (Anolis sagrei))
Specification
Regenerated lizard tail (Anolis sagrei)
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Thomas Lozito

Verified customer

投稿 May 06 2014

Thank you for your phone call today and for your questions about using ab75150 in IHC.

Please keep me updated about the results using this antibody after a heat-mediated antigen retrieval with pH 6 citrate buffer. If you need any further assi...

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