All structural antigens. Cross-reacts with Parainfluenza type 2 but has shown minimal reactivity with Parainfluenza type 1. Does not react with HEp-2 cells by indirect immunofluorescence.
Some cross reactivity with bovine Parainfluenza-3 and canine Parainfluenza.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
Direct FA staining of target antigens in a permissive tissue culture system.
A working dilution must be determined by the user but a starting range of
1:10 - 1:50 is suggested.
Acetone fixation of the antigen source is recommended prior to staining.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Parainfluenza viruses belong to the genus Paramyxovirus of the family Paramyxoviridae. They are enveloped viruses with a single-strand RNA genome of negative polarity and range in diameter from 150 to 200 nm. Four types of parainfluenza viruses have been identified. Types 1 and 2 are major causes of laryngotracheo-bronchitis (croup), with greatest severity in children ages 2 to 4 years. Type 3 infection can also lead to croup but, most notably, is a major cause of infant bronchiolitis, pneumonia, and hospitalization. Infection from type 3 virus is most severe in infants less than 1 year old. Parainfluenza type 4 has been associated only with mild upper respiratory illness in adults and children.
has not yet been referenced specifically in any publications.