製品の概要
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製品名Anti-PAR6 antibody
PAR6 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to PAR6
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由来種Goat
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アプリケーション適用あり: ELISA, IHC-Pmore details
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種交差性交差種: Mouse, Human
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免疫原
Peptide with sequence
GSRIRGDGSGFSL
, from C Terminus of the protein sequence according to NP_058644 -
ポジティブ･コントロール
- Human Brain and Pancreas extracts.
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特記事項GenBank Accession Number – NP_058644.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab6022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | ||
| IHC-P |
IHC-P: Use at a concentration of 5 - 10 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
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組織特異性Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
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配列類似性Belongs to the PAR6 family.
Contains 1 OPR domain.
Contains 1 PDZ (DHR) domain.
Contains 1 pseudo-CRIB domain. -
ドメインThe pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.
The OPR domain mediates interactions with MAP2K5.
The PDZ domain mediates the interaction with CRB3. -
細胞内局在Cytoplasm. Cell membrane. Cell projection > ruffle. Cell junction > tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions.
- Information by UniProt
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参照データベース
- Entrez Gene: 50855 Human
- Entrez Gene: 56513 Mouse
- Omim: 607484 Human
- SwissProt: Q9NPB6 Human
- SwissProt: Q9Z101 Mouse
- Unigene: 112933 Human
- Unigene: 472753 Mouse
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別名
- 0710008C04Rik antibody
- 2610010A15Rik antibody
- Par 6 partitioning defective 6 C elegans homolog alpha antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR6 antibody (ab6022)Ab6022 (10ug/ml) staining human PAR6 in human pancreas by immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.Ab6022 shows strong cytoplasmic staining in rare cells of intralobular ducts.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR6 antibody (ab6022)ab6022 at 5ug/ml staining PAR6 in human heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
参考文献
This product has been referenced in:
- Wu M et al. Epicardial Spindle Orientation Controls Cell Entry into the Myocardium. Dev Cell 19:114-125 (2010). IHC-P ; Mouse . Read more (PubMed: 20643355) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"