製品の概要

  • 製品名Anti-PAR4 antibody [EPR3992]
    PAR4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3992] to PAR4
  • アプリケーション適用あり: WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human PAR4.

  • ポジティブ・コントロール
    • LnCaP, HeLa, Jurkat, A549 cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 37 kDa).
  • 追加情報Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • ターゲット情報

    • 機能Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.
    • 組織特異性Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers.
    • ドメインThe leucine-zipper domain is not essential for apoptosis, but is required for sensitization of cells to exogenous apoptotic insults and for interaction with its partners.
      The SAC domain is a death-inducing domain selective for apoptosis induction in cancer cells. This domain is essential for nuclear entry, Fas activation, inhibition of NF-kappa-B activity and induction of apoptosis in cancer cells.
    • 翻訳後修飾Preferentially phosphorylated at the Thr-163 by PKC in cancer cells.
    • 細胞内局在Cytoplasm. Nucleus. Mainly cytoplasmic in absence of apoptosis signal and in normal cells. Nuclear in most cancer cell lines. Nuclear entry seems to be essential but not sufficient for apoptosis (By similarity). Nuclear localization includes nucleoplasm and PML nuclear bodies.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 2310001G03Rik antibody
      • PAR 4 antibody
      • PAR-4 antibody
      • Pawr antibody
      • PAWR_HUMAN antibody
      • PRKC Apoptosis WT1 Regulator antibody
      • PRKC apoptosis WT1 regulator protein antibody
      • Prostate apoptosis response 4 protein antibody
      • Prostate apoptosis response protein 4 antibody
      • prostate apoptosis response protein PAR-4 antibody
      • Transcriptional repressor Par-4-like protein PAWR antibody
      • Transcriptional repressor PAR4 antibody
      • WT1 Interacting Protein antibody
      see all

    Anti-PAR4 antibody [EPR3992] 画像

    • All lanes : Anti-PAR4 antibody [EPR3992] (ab109174) at 1/1000 dilution

      Lane 1 : LnCaP cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 37 kDa
      Observed band size : 43 kDa (why is the actual band size different from the predicted?)

    Anti-PAR4 antibody [EPR3992] (ab109174) 使用論文

    This product has been referenced in:
    • Chen X  et al. Fbxo45-mediated degradation of the tumor-suppressor Par-4 regulates cancer cell survival. Cell Death Differ 21:1535-45 (2014). Read more (PubMed: 24992930) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"