製品の概要

  • 製品名Anti-PAR4 antibody
    PAR4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PAR4
  • アプリケーション適用あり: WB, IP, ICC, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human PAR4 aa 324-342.
    Sequence:

    EQLKQENKTLLKVVGQLTR


    (Peptide available as ab5853)

  • ポジティブ・コントロール
    • Rat spleen extract. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: A549

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab5787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).Can be blocked with PAR4 peptide (ab5853).
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 19801652
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.
  • 組織特異性Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers.
  • ドメインThe leucine-zipper domain is not essential for apoptosis, but is required for sensitization of cells to exogenous apoptotic insults and for interaction with its partners.
    The SAC domain is a death-inducing domain selective for apoptosis induction in cancer cells. This domain is essential for nuclear entry, Fas activation, inhibition of NF-kappa-B activity and induction of apoptosis in cancer cells.
  • 翻訳後修飾Preferentially phosphorylated at the Thr-163 by PKC in cancer cells.
  • 細胞内局在Cytoplasm. Nucleus. Mainly cytoplasmic in absence of apoptosis signal and in normal cells. Nuclear in most cancer cell lines. Nuclear entry seems to be essential but not sufficient for apoptosis (By similarity). Nuclear localization includes nucleoplasm and PML nuclear bodies.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2310001G03Rik antibody
    • PAR 4 antibody
    • PAR-4 antibody
    • Pawr antibody
    • PAWR_HUMAN antibody
    • PRKC Apoptosis WT1 Regulator antibody
    • PRKC apoptosis WT1 regulator protein antibody
    • Prostate apoptosis response 4 protein antibody
    • Prostate apoptosis response protein 4 antibody
    • prostate apoptosis response protein PAR-4 antibody
    • Transcriptional repressor Par-4-like protein PAWR antibody
    • Transcriptional repressor PAR4 antibody
    • WT1 Interacting Protein antibody
    see all

Anti-PAR4 antibody 画像

  • ICC/IF image of ab5787 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab5787 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.



  • Predicted band size : 41 kDa

    Shows a Western blot of PAR4 on rat spleen extract using ab5787.

  • Immunohistochemical analysis of paraffin-embedded mouse embryonic corneal tissue, staining PAR4 with ab5787.

    Embryos were fixed with 4% paraformaldehyde and treated for antigen retrieval by boiling for 10 min in the citrate buffer (pH 6.0). Sections were incubated with primary antibody and a biotinylated anti-rabbit secondary antibody.

Anti-PAR4 antibody (ab5787) 使用論文

This product has been referenced in:
  • Acharya M  et al. A complex regulatory network of transcription factors critical for ocular development and disease. Hum Mol Genet 20:1610-24 (2011). IP . Read more (PubMed: 21282189) »
  • Fernandez-Marcos PJ  et al. Simultaneous inactivation of Par-4 and PTEN in vivo leads to synergistic NF-kappaB activation and invasive prostate carcinoma. Proc Natl Acad Sci U S A 106:12962-7 (2009). IHC-P ; Human . Read more (PubMed: 19470463) »

See all 4 Publications for this product

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Vielen Dank für Ihre Nachricht.

Es tut mir leid, dass Sie Probleme mit diesem PAR4-Antikörper hatten. Wie besprochen, habe ich eine kostenlose Ersatzlieferung mit ab5787für Sie in Auftrag gegeben. Sie hat die Referenznummer x u...

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Thanks for your call earlier this week and for your patience. The immunogenic peptide for the PAR4 antibody ab5787 is available as ab5853- http://www.abcam.com/PAR4-peptide-ab5853.html You can use this peptide in a blocking assay in order to demonstrat...

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Thank you for your patience. In reviewing the information, we see Western blot with multiple bands at 40 kDa, 38 kDa and unknown at 20 kDa. It is most likely that the preparation, protocol or storage of antibody that is causing the extra bands. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"