The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報ELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 200 kDa under reducing conditions. (predicted molecular weight: 195 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.
組織特異性High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts.
配列類似性Belongs to the peptidase M43B family. Contains 5 Sushi (CCP/SCR) domains.
発生段階Present in serum and placenta during pregnancy; levels increase throughout pregnancy.
翻訳後修飾There appear to be no free sulfhydryl groups.
Differentially placenta 1 expressed protein antibody
EC 22.214.171.124 antibody
IGF dependent IGFBP 4 protease antibody
IGF-dependent IGFBP-4 protease antibody
IGFBP 4ase antibody
Insulin like growth factor dependent IGF binding protein 4 protease antibody
Insulin-like growth factor-dependent IGF-binding protein 4 protease antibody
PAPP A antibody
Pappalysin 1 precursor antibody
Pregnancy Associated Plasma Protein A antibody
Pregnancy associated plasma protein A pappalysin 1 antibody
Pregnancy-associated plasma protein A antibody
Anti-PAPP A antibody [18A10] 画像
Western blot - PAPP A antibody [18A10] (ab10196)
Predicted band size : 195 kDa
Detection of human PAPP-A by ab10196 in Western Blotting. A. ab10196 recognizes PAPP-A subunit after SDS-gel electrophoresis in reducing conditions (gradient gel 5 % – 15 %) B. ab10196 recognizes heterotetrameric complex (gradient gel 2.5 % – 10 %)
Anti-PAPP A antibody [18A10] (ab10196) 使用論文
This product has been referenced in:
Escamilla-Gómez V et al. Simultaneous detection of free and total prostate specific antigen on a screen-printed electrochemical dual sensor. Biosens Bioelectron24:2678-83 (2009).
Read more (PubMed: 19261459) »