製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PAPOLA
  • アプリケーション
    適用あり: WBmore details
    適用なし: IP
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to a region between residue 694 and the C-terminus (residue 744) of human PAPOLA (NP_116021.2)

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab72492 was affinity purified using an epitope specific to PAPOLA immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.
    • 配列類似性
      Belongs to the poly(A) polymerase family.
    • 翻訳後修飾
      Polysumoylated. Varying sumolyation depending on tissue- and cell-type. Highly sumoylated in bladder and NIH 3T3 cells. Sumoylation is required for nuclear localization and enhances PAP stability. Desumoylated by SENP1. Inhibits polymerase activity.
      Hyperphosphorylation on multiple CDK2 consensus and non-consensus sites in the C-terminal Ser/Thr-rich region represses PAP activity in late M-phase. Phosphorylation/dephosphorylation may regulate the interaction between PAP and CPSF.
      Acetylated in the C-terminus. Acetylation decreases interaction with NUDT21 and KPNB1, and inhibits nuclear localization through inhibiting binding to the importin alpha/beta complex.
    • 細胞内局在
      Cytoplasm. Nucleus. The 90 kDa form is nuclear while the 100 kDa and the 106 kDa forms are both nuclear and cytoplasmic.
    • Information by UniProt
    • 参照データベース
    • 別名
      • MGC5378 antibody
      • PAP alpha antibody
      • PAP antibody
      • PAP-alpha antibody
      • PapIII antibody
      • PAPOA_HUMAN antibody
      • PAPOLA antibody
      • Plap antibody
      • Poly(A) polymerase alpha antibody
      • Polynucleotide adenylyltransferase alpha antibody
      see all

    画像

    • All lanes : Anti-PAPOLA antibody (ab72492) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg


      Predicted band size : 83 kDa
      Observed band size : 83 kDa


      Exposure time : 3 minutes

    プロトコール

    参考文献

    This product has been referenced in:
    • Sagawa F  et al. Nucleophosmin deposition during mRNA 3' end processing influences poly(A) tail length. EMBO J 30:3994-4005 (2011). Read more (PubMed: 21822216) »

    See 1 Publication for this product

    レビューと Q&A

    Thank you for the protocol and result details. Regarding a replacement, there have not been other complaints about the antibody but not many people have purchased it. We have only the lot that you received, and I am concerned that another vial will sim...

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    Thank you for your reply. I have found your order in our system.

    Could you please let me know also the following details:

    1) What antibody dilution and incubation time was used?
    2) What was used for blocking?
    3) You repor...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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