製品の概要

  • 製品名Anti-Pannexin 1 antibody [EPR5556]
    Pannexin 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5556] to Pannexin 1
  • アプリケーション適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC/IF or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Pannexin 1 aa 350 to the C-terminus. The exact sequence is proprietary.

  • ポジティブ・コントロール
    • WB: Human fetal brain tissue lysate and SH-SY5Y, Caco2, and NIH OVCAR-3 cell lysates. IP: SH-SY5Y cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 解離定数(KD 値) KD = 1.52 x 10 -10 M Learn more about KD
  • バッファーpH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名EPR5556
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/10 - 1/100.
  • 追加情報Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • ターゲット情報

    • 機能Structural component of the gap junctions and the hemichannels. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis.
    • 配列類似性Belongs to the pannexin family.
    • 細胞内局在Cell membrane. Cell junction > gap junction. Endoplasmic reticulum membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • innexin antibody
      • MRS1 antibody
      • Pannexin 1 antibody
      • Pannexin-1 antibody
      • Panx1 antibody
      • PANX1_HUMAN antibody
      • PX1 antibody
      • UNQ2529 antibody
      see all

    Anti-Pannexin 1 antibody [EPR5556] 画像

    • All lanes : Anti-Pannexin 1 antibody [EPR5556] (ab124969) at 1/10000 dilution (purified)

      Lane 1 : Caco-2 whole cell lysate
      Lane 2 : NIH:OVCAR-3 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 48 kDa
      Observed band size : 48 kDa

      Blocking and dilution buffer: 5% NFDM /TBST.

    • ab124969 (purified) at 1/20 immunoprecipitating Pannexin 1 in SH-SY5Y whole cell lysate.

      Lane 1 (input): SH-SY5Y whole cell lysate (10µg)

      Lane 2 (+): ab124969 + SH-SY5Y whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124969 in SH-SY5Y whole cell lysate.

      For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-Pannexin 1 antibody [EPR5556] (ab124969) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal brain cell lysates
      Lane 2 : SH-SY5Y cell lysates
      Lane 3 : Caco2 cell lysates
      Lane 4 : NIH OVCAR-3 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 48 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Pannexin 1 antibody [EPR5556] (ab124969) 使用論文

    This product has been referenced in:
    • Skals M  et al. Bacterial RTX toxins allow acute ATP release from human erythrocytes directly through the toxin pore. J Biol Chem 289:19098-109 (2014). WB ; Mouse . Read more (PubMed: 24860098) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab124969.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"