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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Pannexin 1 aa 350 to the C-terminus. The exact sequence is proprietary.
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Our Abpromise guarantee covers the use of ab124969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|IP||1/10 - 1/100.|
Blocking and dilution buffer: 5% NFDM /TBST.
ab124969 (purified) at 1/20 immunoprecipitating Pannexin 1 in SH-SY5Y whole cell lysate.
Lane 1 (input): SH-SY5Y whole cell lysate (10µg)
Lane 2 (+): ab124969 + SH-SY5Y whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124969 in SH-SY5Y whole cell lysate.
For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.