製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to PANK1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human PANK1 protein (NP_683879.1)

  • ポジティブ・コントロール
    • PANK1 transfected 293T cell lysate and human liver tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: 1/500 - 1/1000. Detects band of approximately 37 and 50 kDa in two separate blots (predicted molecular weight for isoform 2: 41 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Plays a role in the physiological regulation of the intracellular CoA concentration.
    • 組織特異性
      Isoform 1 is expressed at high levels in brain, heart, kidney, liver, skeletal muscle and testis. Isoform 2 is detected at much lower levels in kidney, liver, brain and testis and is not detected in heart or skeletal muscle.
    • パスウェイ
      Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.
    • 配列類似性
      Belongs to the type II pantothenate kinase family.
    • ドメイン
      The N-terminal extension, present in isoform 1 may be the regulatory domain.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • coaA antibody
      • hPanK antibody
      • hPanK1 antibody
      • PANK antibody
      • PANK1 antibody
      • PANK1_HUMAN antibody
      • PANK1a antibody
      • PANK1b antibody
      • PanK1beta antibody
      • Pantothenate kinase 1 antibody
      • Pantothenate kinase antibody
      • Pantothenic acid kinase 1 antibody
      see all

    画像

    • Anti-PANK1 antibody (ab69354) at 1/500 dilution + human liver tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 41 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-PANK1 antibody (ab69354) at 1/500 dilution

      Lane 1 : PANK1 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 41 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    ab69354 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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