Anti-Pancreatic Polypeptide 抗体 (ab113694)

製品の概要

  • 製品名Anti-Pancreatic Polypeptide antibody
    Pancreatic Polypeptide 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Pancreatic Polypeptide
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Chimpanzee, Macaque Monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Pancreatic Polypeptide.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in FFPE human pancreas tissue sections.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab113694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma levels of this hormone have been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.
  • 細胞内局在Secreted
  • 参照データベース
  • 別名
    • Pancreatic polypeptide antibody
    • Pancreatic polypeptide Y antibody
    • Pancreatic prohormone antibody
    • PNP antibody
    • PP antibody
    • PPY antibody
    • prepro-PP (prepropancreatic polypeptide) antibody
    see all

Anti-Pancreatic Polypeptide antibody 画像

  • IHC image of Pancreatic Polypeptide staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113694, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Pancreatic Polypeptide antibody (ab113694) 使用論文

ab113694 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step hydrogen peroxide as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: LeicaBond epitope retrieval solution 1
Sample Rat Tissue sections (Pancreas, Islets of Langerhans, PP-cells)
Specification Pancreas, Islets of Langerhans, PP-cells
Permeabilization No
Fixative Formaldehyde
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投稿 Dec 05 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"