Anti-Pancreatic alpha amylase 抗体 (ab21156)

製品の概要

  • 製品名Anti-Pancreatic alpha amylase antibody
    Pancreatic alpha amylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Pancreatic alpha amylase
  • 特異性Cross-reactivity against other forms of amylase has not been determined.
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Full length native protein (purified) corresponding to Human Pancreatic alpha amylase.

  • ポジティブ・コントロール
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal pancreas

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab21156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/8000. Predicted molecular weight: 57 kDa. Strong reaction to 0.15ug and 0.3ug antigen detected. Predicted molecular weight: 57 kDa. Dilution optimised using Chromogenic detection.
IHC-P 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性Pancreatic amylase is a digestive enzyme secreted by the pancreas. It consists of a single polypeptide chain with a molecular weight of 54 kDa. Pancreatic amylase occurs in serum at a concentration of about 60µg/L, from a total amylase concentration in serum of about 120µg/L. Pancreatic amylase and salivary amylase are the products of separate genes and, although they share 98% amino acid sequence homology, they differ in molecular size, isoelectric point and antigenic properties. Measurement of amylase concentrations in serum is a diagnostic test for various pancreatic disorders (especially acute and relapsing pancreatitis).
  • 細胞内局在Secreted protein; extracellular space.
  • 参照データベース
  • 別名
    • 1 4 alpha D glucan glucanohydrolase antibody
    • 1,4 alpha D glucan glucanohydrolase 2B antibody
    • Alpha amylase 2B antibody
    • Alpha amylase antibody
    • AMY2 antibody
    • AMY2A antibody
    • AMY2B antibody
    • AMY3 antibody
    • Amylase alpha 2A antibody
    • Amylase alpha 2A pancreatic antibody
    • Amylase alpha 2B antibody
    • Amylase alpha 2B pancreatic antibody
    • Amylase pancreatic alpha 2A antibody
    • Amylase pancreatic alpha 2B antibody
    • Amylase, alpha 2A (pancreatic) antibody
    • Amylase, alpha 2B (pancreatic) antibody
    • Carcinoid alpha amylase antibody
    • Found in the pancreas antibody
    • Glycogenase antibody
    • HXA antibody
    • PA antibody
    • Pancreatic alpha amylase antibody
    • Pancreatic amylase 2A antibody
    • Pancreatic amylase alpha 2A antibody
    see all

Anti-Pancreatic alpha amylase antibody 画像

  • IHC image of Pancreatic Amylase staining in Human pancreas normal formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21156, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Pancreatic alpha amylase antibody (ab21156) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Permeabilization No
Fixative no
Username

Mrs. Sandy Piampiano

Verified customer

投稿 Feb 20 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas)
Specification pancreas
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid buffer
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Serum (serum)
Loading amount 30 µg
Specification serum
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 24 2012

Thank you for your inquiry.

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Thank you for contacting us. Generally we predict reactivity of an antibody if the immunogen sequence has 85% or greater homology with the species being tested. The immunogen for ab35414 and ab82890 is full length pig protein and for ab21156 it is full...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"