製品の概要

  • 製品名Anti-Pan3 antibody
    Pan3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Pan3
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human Pan3.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell; HepG2 Whole Cell; MOLT4 Whole Cell; Jurkat Whole Cell; Human Liver Tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 82 kDa).

ターゲット情報

  • 機能Functions in cytoplasmic mRNA decay. As part of the Pan nuclease complex, recruits polyadenylate-binding protein which in turn stimulates PAN2 nuclease activity.
  • 配列類似性Belongs to the protein kinase superfamily.
    Contains 1 protein kinase domain.
  • ドメインThe protein kinase domain is predicted to be catalytically inactive.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hPan3 antibody
    • PAB dependent poly(A) specific ribonuclease subunit 3 antibody
    • PAB-dependent poly(A)-specific ribonuclease subunit 3 antibody
    • PAB1P dependent poly(A) nuclease antibody
    • PABP dependent poly(A) nuclease 3 antibody
    • PABP1 dependent poly A specific ribonuclease subunit PAN3 antibody
    • Pan 3 antibody
    • PAN deadenylation complex subunit 3 antibody
    • PAN3 antibody
    • PAN3 poly(A) specific ribonuclease subunit antibody
    • PAN3 poly(A) specific ribonuclease subunit homolog antibody
    • PAN3 polyA specific ribonuclease subunit homolog antibody
    • PAN3_HUMAN antibody
    see all

Anti-Pan3 antibody 画像

  • All lanes : Anti-Pan3 antibody (ab88642) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 82 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 160 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

Anti-Pan3 antibody (ab88642) 使用論文

ab88642 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Neuroblastoma cell line N2A)
Loading amount 30 µg
Specification Mouse Neuroblastoma cell line N2A
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jul 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (HeLa)
Loading amount 40 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (7)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 13 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

投稿 Mar 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"