製品の概要

  • 製品名Anti-pan Synuclein antibody
    pan Synuclein 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to pan Synuclein
  • 特異性Detects endogenous levels of total Synuclein protein. This antibody recognises all forms of synuclein (alpha, beta and gamma).
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide within Human pan Synuclein aa 15-64. The exact sequence is proprietary. Synthetic peptide derived from human synuclein.

  • ポジティブ・コントロール
    • WB: Extracts from LOVO cells. IHC-P: Human brain tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

ターゲット情報

  • 関連性Synucleins are small, soluble proteins expressed primarily in neural tissue and in certain tumors. The family includes three known proteins: alpha, beta and gamma synucleins. Synuclein-alpha is primarily localized at the presynaptic region of axons and it may be involved in the regulation of dopamine release and transport. However, it can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Synuclein-alpha may induce fibrillization of the microtubule-associated protein tau and it reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. Synuclein-alpha is involved in Parkinson's disease, Lewy body dementia, Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome) and it is a major non-amyloid component of the deposits associated with Alzheimer's disease. Synuclein-beta may be a regulator of the SNCA aggregation process. It protects neurons from staurosporine and 6 hydroxy dopamine (6OHDA)-stimulated caspase activation that also involves p53 and contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Gamma synuclein is found primarily in the peripheral nervous system and retina. High levels of expression have been identified in advanced breast carcinomas suggesting a correlation between overexpression of SNCG and breast tumor development.
  • 細胞内局在Neuronal cytosol and presynaptic terminals
  • 参照データベース
  • 別名
    • BCSG1 antibody
    • beta synuclein antibody
    • Breast cancer specific gene 1 protein antibody
    • Gamma synuclein antibody
    • NACP antibody
    • PARK1 antibody
    • Persyn antibody
    • PRSN antibody
    • SNCA antibody
    • SNCB antibody
    • SNCG antibody
    • SR antibody
    • Synoretin antibody
    see all

Anti-pan Synuclein antibody 画像

  • All lanes : Anti-pan Synuclein antibody (ab53726) at 1/500 dilution

    Lane 1 : rat heart lysate
    Lane 2 : rat heart lysate with immunizing peptide

    Lysates/proteins at 5 µg per lane.

    Secondary
    Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
  • Ab53726 at 1/50 dilution, staining paraffin embedded human brain in the presence (right) or absence (left) of blocking peptide.

Anti-pan Synuclein antibody (ab53726) 使用論文

This product has been referenced in:
  • Busch DJ  et al. Acute increase of a-synuclein inhibits synaptic vesicle recycling evoked during intense stimulation. Mol Biol Cell 25:3926-41 (2014). Read more (PubMed: 25273557) »
  • Lau BY  et al. Cyclic AMP promotes axon regeneration, lesion repair and neuronal survival in lampreys after spinal cord injury. Exp Neurol 250C:31-42 (2013). WB, IHC-P . Read more (PubMed: 24041988) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Sample Lampreys (Petromyzon marinus) Tissue lysate - whole (Brain and spinal cord protein extracts)
Specification Brain and spinal cord protein extracts
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Jennifer Morgan

Verified customer

投稿 Feb 26 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Sample Lampreys (Petromyzon marinus) Cell (spinal cord cryosections)
Specification spinal cord cryosections
Permeabilization Yes - Triton X-100
Fixative Paraformaldehyde
Username

Jennifer Morgan

Verified customer

投稿 Feb 26 2015

Thank you for your enquiry. We are not aware of anyreferences for this antibody, and the only data we have is what is on the datasheet. You may want to consider the antibody ab6176. The datasheet lists six references.

http://www.abcam.com/ind...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"