Anti-pan PDK 抗体 [3H5BE12] (ab115321)

製品の概要

  • 製品名Anti-pan PDK antibody [3H5BE12]
  • 製品の詳細
    Mouse monoclonal [3H5BE12] to pan PDK
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein

  • 特記事項

     

    PDH Kinases (PDKs) deactivate PDH by reversible phosphorylation. In humans and rodents, 4 PDK's are present in an organ-dependent manner. In all cases, PDK's are physically part of the PDH complex (binding via lipoyl domain 2 of E2), and they phosphorylate the E1 subunit at 3 specific serine residues. PDH phosphatases remove the phosphorylation and restore activity of PDH.

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa.

Anti-pan PDK antibody [3H5BE12] 画像

  • All lanes : Anti-pan PDK antibody [3H5BE12] (ab115321) at 1 µg/ml

    Lane 1 : human heart mitochondria at 20 µg
    Lane 2 : human liver mitochondria at 10 µg
    Lane 3 : rat liver mitochondria at 10 µg
    Lane 4 : mouse liver mitochondria at 10 µg
    Lane 5 : PDK1 recombinant protein ab110359 at 0.1 µg
    Lane 6 : PDK2 recombinant protein ab110354 at 0.1 µg
    Lane 7 : PDK3 recombinant protein ab110355 at 0.1 µg
    Lane 8 : PDK4 recombinant protein ab110356 at 0.1 µg
    Lane 9 : PDK1 recombinant protein ab86986 at 0.1 µg

    Secondary
    GAM IR 800 1:5000

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    ab115321 identifies all four isoforms of PDKs (PDK1, PDK2, PDK3, and PDK4) with differential affinity. PDK3 has the highest affinity, then PDK2 and 4, it has the lowest affinity to PDK1.
  • IHC image of ab115321 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab115321, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 49 kDa


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    Western blot analysis of human ovarian cancer cell lines whole cell lysate (30µg/lane) labelling pan PDK with ab115321 at 1/1000. A HRP-conjugated goat anti-mouse IgG polyclonal (1/10000) was used as the secondary antibody.

    See Abreview

Anti-pan PDK antibody [3H5BE12] (ab115321) 使用論文

ab115321 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (prostate cancer cell line)
Gel Running Conditions Reduced Denaturing (10% acrylamide gel)
Loading amount 20 µg
Specification prostate cancer cell line
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Stamatia Pouliliou

Verified customer

投稿 Jun 26 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (U87MG Human Glioma)
Specification U87MG Human Glioma
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

投稿 Feb 27 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-15% GRADIENT GEL)
Sample Human Cell lysate - whole cell (OVARIAN CANCER CELL LINES)
Specification OVARIAN CANCER CELL LINES
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 30 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HeLa, U2OS: Purified mitochondria lysate)
Loading amount 2e+006 cells
Specification HeLa, U2OS: Purified mitochondria lysate
Gel Running Conditions Reduced Denaturing (8% polyacrylamide gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Aug 09 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"