The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
IHC-Fr: 1/100. 2% paraformaldehyde-lysine / sodium periodate or acetone fixation.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The antigen is found on a subpopullation of most tissue fixed macrophages. It is quite predominant on red pulp macrophages and interdigitating cells in the outer periarteriolar lymphatic sheath of the spleen, the inner border of marginal sinus of lymph nodes, and alveolar macrophages. Langerhans cells of the skin and twisted spindle-shaped cells of the omentum are detected by the antibody. It is absent from sinusoidal endothelial cells and parenchymal cells.