Anti-pan Cytokeratin 抗体 [C-11] (Phycoerythrin) (ab52460)


  • 製品名Anti-pan Cytokeratin antibody [C-11] (Phycoerythrin)
    pan Cytokeratin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [C-11] to pan Cytokeratin (Phycoerythrin)
  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特異性ab52460 recognises Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    Reacts with all species (recognized epitope conserved within all species)
  • 免疫原

    Keratin-enriched preparation from human epidermoid carcinoma cell line A431.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Prior to conjugation, this antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
  • ポリ/モノモノクローナル
  • クローン名C-11
  • アイソタイプIgG1
  • 研究分野


Our Abpromise guarantee covers the use of ab52460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1-2µl for 106 cells.

(in a 100µl cell suspension).

ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 関連性Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • 細胞内局在Cytoplasmic

Anti-pan Cytokeratin antibody [C-11] (Phycoerythrin) 画像

  • Intracellular flow cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using ab52460.

    Recommended conc. 0.5 μg/ml

    Overlay with Isotype mouse IgG1 control.

Anti-pan Cytokeratin antibody [C-11] (Phycoerythrin) (ab52460) 使用論文

This product has been referenced in:
  • Rajendran S  et al. Preclinical evaluation of gene delivery methods for the treatment of loco-regional disease in breast cancer. Exp Biol Med (Maywood) 236:423-34 (2011). Read more (PubMed: 21444371) »
  • Rajendran S  et al. Targeting of breast metastases using a viral gene vector with tumour-selective transcription. Anticancer Res 31:1627-35 (2011). Read more (PubMed: 21617219) »

See all 3 Publications for this product

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We have a PE- conjugated antibody against cytokeratin, catalogue item ab52460:

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