Anti-pan Cytokeratin 抗体 [AE1/AE3] (ab27988)

製品の概要

  • 製品名
    Anti-pan Cytokeratin antibody [AE1/AE3]
    pan Cytokeratin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AE1/AE3] to pan Cytokeratin
  • 特異性
    Ab27988 recognises Pan cytokeratin.
  • アプリケーション
    適用あり: IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human, Pig, Macaque monkey
  • 免疫原

    Full length protein: Human epidermal keratins.

  • ポジティブ・コントロール
    • Human skin, oesophagus.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.1% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    モノクローナル
  • クローン名
    AE1/AE3
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab27988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/10 - 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

This product requires protein digestion pretreatment (e.g. trypsin or pronase), or antigen retrieval using heat treatment prior to staining of paraffin sections.

IHC-Fr 1/10 - 1/40.

ターゲット情報

  • 関連性
    Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • 細胞内局在
    Cytoplasmic
  • 参照データベース

Anti-pan Cytokeratin antibody [AE1/AE3] 画像

  • Ab27988 at 1/10 dilution staining formalin fixed paraffin embedded oesophagus by Immunohistochemisty.
  • ab27988 staining Cytokeratin in Mouse tissue sections (Human tumor xenograft (Breast carcinoma)) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with MOM kit from Vector Laboratories for 1 hour at 15°C; antigen retrieval was by enzymatic in proteinase K. Samples were incubated with primary antibody (1/20) for 30 minutes at 15°C. A Biotin-conjugated Goat anti-mouse polyclonal (1/10) was used as the secondary antibody.

    See Abreview

プロトコール

Anti-pan Cytokeratin antibody [AE1/AE3] (ab27988) 使用論文

This product has been referenced in:
  • Crowley C  et al. Surface modification of a POSS-nanocomposite material to enhance cellular integration of a synthetic bioscaffold. Biomaterials 83:283-93 (2016). IHC-P ; Human . Read more (PubMed: 26790147) »
  • Rydell-Törmänen K  et al. Aberrant nonfibrotic parenchyma in idiopathic pulmonary fibrosis is correlated with decreased ß-catenin inhibition and increased Wnt5a/b interaction. Physiol Rep 4:N/A (2016). IHC-P ; Human . Read more (PubMed: 26997628) »

See all 11 Publications for this product

Product Wall

The concentration of this lot of ab27988 Anti-pan Cytokeratin antibody [AE1/AE3] is 80 µg/ml.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Human tumor xenograft (Breast carcinoma))
Specification
Human tumor xenograft (Breast carcinoma)
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K at 37C for 15min
Permeabilization
No
Blocking step
Used MOM Kit from Vector Laboratories as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 15°C
Username

Abcam user community

Verified customer

投稿 Jul 31 2012

Thank you for contacting Abcam regarding ab27988.


The antigen retrieval buffer we would recommend for this antibody is for Tris/EDTA.


I hope this information is helpful. Please do not hesitate to contact me if you have any a...

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Thank you for contacting us with your question.

I have looked throughourrecords, and this antibody has actually been tested with mouse samples and it does cross-react with this species. So, it will not be suitable for your assay, and unfortun...

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Thank you for your inquiry.

For ab758, the immunogen that was used was cytoskeletal extract of human hepatocellular carcinoma cell line, Hep3B. We have not validated this antibody in pig. But the human sequence is 85% homologous with the pig ...

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Thank you for your enquiry and your interest in our products.

I can confirm that neither of these antibodies has been tested for cross-reactivity with mouse. If you wish to test one of them in mouse system, I could offer you a testing discoun...

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Thanks for your enquiry.

For this antibody, the exact amount of specific antibody present in certain antibody preparations is not measured. The approximate concentration range for this product is 25 - 200 ug/ml.

I hope this is helpf...

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Thank you for your enquiry.

This antibody has been succesfully tested to react with formalin fixed sections. As far as we know ab27988 has not yet been tested for reactivity to rat so we have no data to suggest if it will or will not detect r...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin, epidermis)
Specification
skin, epidermis
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH 8.0
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Mr. Erwin Kump

Verified customer

投稿 Aug 19 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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