Anti-pan Cytokeratin 抗体 [5D3 + LP34] (ab17153)

製品の概要

  • 製品名Anti-pan Cytokeratin antibody [5D3 + LP34]
    pan Cytokeratin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5D3 + LP34] to pan Cytokeratin
  • 特異性This antibody stains cytokeratin 5,6,8 and 18 and has been designed for a broad spectrum screening of cytokeratins. It stains carcinomas of simple and squamous origin.
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Cat, Human
  • 免疫原

    Cytokeratins from human breast carcinoma cell line MCF-7 and detergent insoluble fraction of psoriatic epidermis.

  • ポジティブ・コントロール
    • Skin.
  • 特記事項This antibody is a cocktail of two different clones; 5D3 (IgG2a) and LP53 (IgG1).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab17153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-P 1/15 - 1/50. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

ターゲット情報

  • 関連性Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
  • 細胞内局在Cytoplasmic
  • 参照データベース

Anti-pan Cytokeratin antibody [5D3 + LP34] 画像

  • ab17153 staining pan Cytokeratin (green) in Human cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Tween 20 and blocked with 10% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. ab150113, a goat anti-mouse Alexa Fluor®488 (IgG polyclonal; 1/100) was used as the secondary antibody.

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  • ICC/IF image of ab17153 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-pan Cytokeratin antibody [5D3 + LP34] (ab17153) at 1/500 dilution ((in 5% Marvel TBS-T). Incubation for 1 hour at 22°C) + Whole cell lysate of human HT1080 cells at 25 µg

    Secondary
    An HRP-conjugated Sheep anti-mouse IgG monoclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 15 minutes

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at 22°C

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  • ab17153 staining human skin and lung sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab17153 at 1/250 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary antibody.

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  • ab17153 staining pan Cytokeratin in formalin-fixed, paraffin-embedded Human skin tissue by Immunohistochemistry. Staining was detected using DAB.

Anti-pan Cytokeratin antibody [5D3 + LP34] (ab17153) 使用論文

ab17153 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Pig Tissue sections (Small intestine)
Specification Small intestine
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Nov 03 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Chicken Tissue sections (Lung)
Specification Lung
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jan 15 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Cat Tissue sections (Lung)
Specification Lung
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jan 14 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Goat Tissue sections (Lung)
Specification Lung
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jan 13 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Dog Tissue sections (Lung)
Specification Lung
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jan 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Human Cell (mammalian cancer cells)
Specification mammalian cancer cells
Permeabilization Yes - Tween20
Fixative Formaldehyde
Username

Mr. Gabriel Ortega

Verified customer

投稿 Jul 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (a549 lung cancer)
Specification a549 lung cancer
Fixative Paraformaldehyde
Permeabilization Yes - triton .25%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 Apr 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung and skin)
Specification Lung and skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Mar 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 cell line)
Loading amount 25 µg
Specification NIH 3T3 cell line
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jun 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HT1080)
Loading amount 25 µg
Specification HT1080
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jun 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"